苯并[a]芘单独以及与雌二醇联合染毒对小鼠肺组织雌激素受体-β表达的影响  

The Expression of Estrogen Receptor Beta in the Lung Tissue of Mice Treaded with Benzo [a]pyrene Alone or Combined with Estrogen

作　　者：张云霄[1] 郑宇飞[2] 陈照立[3] 杨杰[4] 谌志强[3] 王新为[3] 金敏[3] 王晟怡[1] 赵国范[1] 李君文[3] 

机构地区：[1]天津医科大学基础医学院,天津300700 [2]吉林大学公共卫生学院,吉林长春130021 [3]军事医学科学院卫生学环境医学研究所,天津300050 [4]中国疾病预防控制中心,北京100050

出　　处：《环境与健康杂志》2009年第4期293-296,共4页Journal of Environment and Health

摘　　要：目的研究苯并[a]芘(benzo[a]pyrene,B[a]P)单独以及与雌二醇联合染毒对小鼠肺组织雌激素受体-β(estrogen receptor,ER-β)表达的影响,探讨雌激素在B[a]P所致小鼠肺癌中的作用。方法雌性昆明种小鼠125只,随机分为5组,每组25只,正常对照组(橄榄油灌胃和皮下注射)、雌二醇组(900μg/kg雌二醇)、B[a]P组(75μmol/kgB[a]P),B[a]P+高剂量雌二醇组(75μmol/kgB[a]P和900μg/kg雌二醇)和B[a]P+低剂量雌二醇组(75μmol/kgB[a]P和300μg/kg雌二醇),B[a]P灌胃,雌二醇皮下注射,灌胃和注射每周一次,持续8周,之后给予8周的恢复期,共16周。手术切除全肺,采用实时定量PCR(Realtime-PCR)和蛋白质印迹(Westernblotting)技术测定小鼠肺组织中ER-β的表达水平。结果B[a]P组和B[a]P+低剂量雌二醇组ER-β基因和蛋白的表达量明显高于正常对照组,差异有统计学意义(P<0.05)。雌二醇组ER-β基因表达高于正常对照组,差异有统计学意义(P<0.05)。B[a]P+高剂量雌二醇组和雌二醇组中ER-β蛋白表达量明显低于B[a]P组,差异有统计学意义(P<0.05)。结论在本研究剂量范围内,随着雌激素剂量的增加,B[a]P和雌二醇联合作用使ER-βmRNA水平和ER-β蛋白表达下调。ER-β途径在雌激素促进B[a]P致肺癌的剂量-反应关系中的作用有待进一步研究。Objective To study the expression of estrogen receptor beta in the lung tissue of mice treaded with B[a]p alone or combined with estrogen （17 β-estradiol, E2） in female Kunming strain mice and to explore the effect of estrogen in the lung cancer of mice induced by benzo[a]pyrene （B[a]P）. Methods One hundred and twenty-five female Kunming mice were randomly divided into 5 groups： normal control group was group 1 ;group 2 was treated with subcutaneous injection of estrogen （ 900μg/kg）; group 3 was given B [a]P（75μmol/kg） by gavage;the last two groups were given the same dosage of B[a]P （75 μmol/kg）plus two different dosage of estrogen： 900μg/kg and 300μg/kg. After 8 weeks,there was a recovery period of 8 weeks. Then, the lung tissue was obtained by surgical resection. The expression of estrogen receptor-β gene and estrogen receptor-β protein of the lung tissue was detected by real-time PCR and Western blotting technology respectively. Results The expression of estrogen receptor-β protein and estrogen receptor-β gene in the B [a] P group and the B [a]P plus low dosage estrogen group was significantly higher than the normal group （P〈0.05） . The expression of estrogen reeeptor-β gene in the estrogen group was significantly higher than the normal group. The expression of estrogen receptor-β protein in the B[a]P plus high dosage estrogen group and the estrogen group was significantlylower than the B[a]P group （P〈O.05）. Conclusion In the dose range ofour study, the expression ofestrogen receptor-β protein and estrogen receptor-β gene declined with the increase of estrogen. The mechanism needs further exploration.

关 键 词：呼吸系统 苯并[A]芘 雌二醇 雌激素受体-Β 

分 类 号：R994.6[医药卫生—毒理学]