用两种PCR方法对HIV-1测定的敏感度对比分析  

Sensitive analysis on HIV-1 determined by 2 PCR methods

作　　者：张艳敏[1] 孙国清 鲍玉洲[1] 高葆真 张郑民[1] 李建新[1] 

机构地区：[1]河南省眼科研究所分子生物室,河南郑州450003 [2]河南省CDC性病,HIV研究所,河南郑州450016

出　　处：《河南医学研究》2009年第1期9-11,共3页Henan Medical Research

基　　金：河南省科技攻关项目(编号0624410059)

摘　　要：目的：探讨用Roche公司生产的Combas Amplicor HIV-1病毒载量检测试剂与巢式RT-PCR法检测HIV-1这两种检测方法之间的相关性。方法：将81份HIV-1阳性患者血浆样本首先用Combas Amplicor病毒载量检测试剂盒检测出病毒拷贝数／ml，根据病毒拷贝数对数／ml的多少分为5组。再用巢式RT-PCR法对以上样本进行重复测定，观察以上2种检测方法的相关性。结果：用巢式RT—PCR法对用Combas Amplicor病毒载量检测的病毒拷贝数／ml分为2．000～2．999组；3．000～3．999组；4．000～4．999组；5．000—5．999组和〉6．000组病毒拷贝数／ml的检测阳性率分别为87．5％（7／8），93．75％（30／32），95．83％（23／24），100％（14／14）和100％（3／3）。结果表明，随着病毒拷贝数／ml的增高巢式法检测的阳性率也相应增高。结论：巢式RT—PCR由于具有操作简单，价格便宜等优点可作为AIDS的初步筛查，指导抗AIDS药物治疗等使用，对于基层而言是一种简便易行的方法。Objective ： To investigate the relativity between quantitative detection of HIV-1 by Combas Amplicor virus load kit produced by Roche Company and qualitative detection of HIV-1 through nested RT-PCR method. Methods： 81 HIV-1 blood samples were obtained from HIV areas in Henan, first tested viral load copies/ml with quantitative Combas Amplicor, divided into 5 groups according to viral copies/ml then, re-tested above data by nested RT-PCR, observe the coincidence rate between this 2 Methods. Results： Compas Amplicor viral copies/ml as follows, 2. 000-2. 999 group; 3. 000-3. 999 group; 4. 000-4. 999 group; 5. 000-5. 999 group and above 6. 000 group, while each of the number of positive rate were 87.5% （7/8） ,93.75% （30/32） , 95.83% （23/24）,100% （14/14） and 100% （3/3）. The results indicate, with the viral copies/ ml become higher, the positive rate will be raise by nested RT-PCR. Conclusion： The teginning of period tested AIDS by nested RT-PCR, can guide therapy of at-AIDS, furthermore, it has advavtage of process simple, price inexpensive ,for the basic unit it is a method which can carry out.

关 键 词：艾滋病患者 HIV-1病毒载量 聚合酶链式反应 

分 类 号：R512.91[医药卫生—内科学]