柿叶黄酮对大鼠急性前脑缺血再灌注损伤的保护作用  

Neuroprotective Effect of Flavonoid from the Leaves of Diospyros Kaki on Acute Forebrain Ischemia Reperfusion Injury in Rats

作　　者：贝伟剑[1] 李楚源[2] 王德勤[2] 彭文烈[3] 朱心红[4] 

机构地区：[1]广东药学院中医药研究院 [2]广州白云山和记黄埔中药有限公司,510515 [3]中山大学生命科学学院,510275 [4]南方医科大学生理教研室,510515

出　　处：《广西中医药》2009年第2期56-59,共4页Guangxi Journal of Traditional Chinese Medicine

基　　金：广州市科技局2002年度重点科技攻关项目(广州市名优中成药中药现代化研究专项资金资助:2002Z2-E5081)

摘　　要：目的:研究柿叶黄酮对大鼠急性前脑缺血再灌注损伤的保护作用。方法:采用大鼠Pulsinelli四血管阻断方法造成前脑缺血再灌注损伤模型,予柿叶黄酮不同剂量灌胃给药12天,每天2次,实验大鼠分别在造模后第8天处死,取大鼠脑海马组织制作石蜡切片,光镜观察脑海马神经细胞及组织形态学变化。结果:柿叶黄酮高、中、低剂量均能提高大鼠急性前脑缺血再灌注损伤后活CA1锥体神经细胞密度,与模型组比较有显著性差异(P<0.01)。结论:柿叶黄酮对大鼠急性前脑缺血再灌注损伤的神经元具有保护作用。Object：To investigate the neuroprotective effect of Flavonoid from the leaves of Diospyros Kaki （FLDK） on acute forebrain ischemia reperfusion injury in rats. Methods： The forebrain ischemia reperfusion injury model was produced by Pulsinelli＇s fourvessel occlusion in rats, different dosages of Flavonoid from the leaves of Diospyros Kaki were intragastric administrated, twice a day for 12 days in total, the rats were separately sacrificed on the eighth day after modeling, and the rats＇ cerebral hippocampus were taken out for paraffin processing, the cerebral hippocampus neurons and histological changes were observed by light microscope. Results： Flavonoid from the leaves of Diospyros Kaki with high, medium and low dosage could improve the active CAlvertebral neurons density after acute forebrain ischemia reperfusion injury in rats, with a significant difference in comparison with those in the model group （P〈0.01）. Conclusion： Flavonoid from the leaves of Diospyros Kaki has the neuroprotective effect on acute forebrain ischemia reperfusion injury in rats.

关 键 词：柿叶黄酮 前脑缺血再灌注 CA1锥体神经细胞 迟发性神经元死亡 

分 类 号：R285.5[医药卫生—中药学]