玉米亲本间蛋白水解酶差异表达分析初探  

Study on the Differential Expression of the Proteolytic Enzymes Between Filial Generation of Maize and Parents

作　　者：姬生栋[1] 陈鹏[1] 可巧艳 薛华政[3] 王加传[1] 袁召[1] 岳春辉[1] 王知丰[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]新乡市种子公司,河南新乡453000 [3]河南省农业科学院,郑州450002

出　　处：《玉米科学》2009年第2期73-76,共4页Journal of Maize Sciences

基　　金：河南省自然科学基金项目(082300430360);河南省国际科技合作项目(0646620016)

摘　　要：采用复性电泳技术,研究了杂交玉米洛玉23与亲本间蛋白水解酶在pH4.5、7.0、8.5条件下的表达差异。结果表明:①杂交F1代和双亲在蛋白水解酶的种类和酶活上差异显著,比双亲多检测到15、16、18、19、26、31、54、73、113kD9条新酶带。②45kD酶带在玉米的叶片和叶鞘中均检测到,40kD酶带仅在叶片中检测到,可能是跟叶片的发育调控和叶片中众多功能的调控有关。40kD和45kD酶带在酸性、中性、碱性条件下均检测到,以pH8.5时表达最强。③杂交F1代叶片中的蛋白水解酶在不同pH下酶带表达存在差异,pH7.0比pH4.5多检测到16、18、19、31、73kD5条新酶带;pH8.5比pH7.0多检测到15、54、218kD3条酶带,pH8.5新出现的3条酶带很可能是位于叶绿体或细胞质内的碱性蛋白水解酶,可能参与Rubisco酶的调控。Renaturation electrophoresis was used to analyzed the properties and types of proteolytic enzymes on the condition of pH 4.5, pH 7.0 and pH 8.5 between Luoyu-23 and its parents during trefoil stage leaves and leaf sheaths. The results show that the properties and types of proteolytic enzymes were significantly different between F1 and its parents. F1 had detected nine novel bands of 15, 16, 18, 19, 26, 31, 54, 73 and 113 kD and the functions of these novel bands may have some relationship with its heterosis. The 45 kD maybe one of important band in the maize. The 40 kD was a differential band between leaves and leaf sheaths and it may one of vital band in the leaves of maize. 40 kD may relate to the leaf developmental regulation and other functions regulation in leaf of maize. The two vital proteins band 40 and 45 kD may both constitute of three different types and the most important type was the alkaline proteins. Expression of proteolytic enzymes at three different pH conditions was significantly different. At condition of pH 7.0 we detected 16, 18, 19, 31 and 73 kD novel band compared to pH 4.5 and three novel band, 15, 54 and 218 kD, were detected at condition of pH 8.5 compared to pH 7.0. The three novel bands at pH 8.5 may be constituted of alkaline proteins which located in the chloroplast or cytoplasm maybe refer to the regulation of Rubisco.

关 键 词：玉米 蛋白水解酶 复性电泳 

分 类 号：S513.01[农业科学—作物学]