淇河鲫生长激素全长cDNA的克隆与序列分析  

Cloning and Sequencing of Full Length Growth Hormone cDNA from Carassius auratus gibelio var

作　　者：高春生[1,2] 范光丽[2] 杨国宇[1] 裴淑丽[1] 王艳玲[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]西北农林科技大学动科学院,陕西杨凌712100

出　　处：《应用与环境生物学报》2009年第2期211-214,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.39470827);河南省杰出青年科学基金项目(No.970203011)资助~~

摘　　要：采用RT-PCR法和3’,5’RACE(Rapid amplification of cDNA ends)法从淇河鲫脑垂体RNA克隆出生长激素(Growth hormone,GH)cDNA.此cDNA全长1191nt[含Poly(A)14nt],其5’端非编码区长55nt,阅读框(Open reading frame,OAF)长633nt,3’端非编码区长503nt.由其推导的GH前体由210个氨基酸组成,其中氮端前22个氨基酸为信号肽部分.氨基酸序列比较表明,淇河鲫与同目的鲫鱼、鲤鱼、团头鲂和斑马鱼的同源性分别为98.6%、96.2%、91.5%和88.6%;与不同目的胡子鲇和鳗鲡的同源性分别为74.6%和49.5%;与哺乳类的家鼠和人等的同源性低于40%.The full length cDNA encoding growth hormone of Carassius auratus gibelio var was cloned from pituitary RNA with RT-PCR, 3＇and 5＇ RACE （Rapid amplification of cDNA ends）. The GH cDNA, about 1 191 nt （nucleotide） long [including a 14 nt poly（A） tail], consisted of a open reading frame of 633 nt long, and 5＇ and 3＇ untranslated regions of 55 nt and 503 nt long, respectively. The DNA sequence analysis showed that the pregrowth hormone peptide of 210 aa deduced from CagvGH cDNA included a putative signal peptide （22 aa） located in its N-terminal. Homological comparison among CagvGH aa and other species growth hormones showed that the homogeneities were 98.6%, 96.2%, 91.5%, 88.6%, 74.6% and 49.5% compared with those of Carassius auratus, Cyprinius carpio, Megalobrama amblycephala, Danio rerio, Clarias batrachus and Anguilla japonica, respectively, but less than 40% compared with those of Mus musculus and Homo sapiens. Fig 3, Ref 30

关 键 词：生长激素CDNA RT-PCR RACE 淇河鲫 

分 类 号：Q785[生物学—分子生物学]