低弹性模量钛合金不同表面微形态对兔腱膜成纤维细胞生物学行为的影响  

Effect of Low Modulus Titanium Alloy's Different Surface Microtopograph on the Biological Behavior of Rabbits' Lacertus Fibroblast

作　　者：袁超凡[1] 郭征[1] 李靖[1] 张涌泉[1] 付军[1] 沙漠[1] 石磊[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032

出　　处：《生物医学工程学杂志》2009年第2期342-346,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30471754)

摘　　要：评价不同表面微形态Ti-Nb-Zr-Sn系钛合金对兔腱膜成纤维细胞生物学行为及软组织整合的影响。采用组织块法培养原代兔跟腱腱膜成纤维细胞并传代后,接种到光滑对照组和喷砂、微沟嵴和螺纹三种不同表面形态的实验用钛合金薄片上,通过MTT比色法、扫描电镜等技术,观察细胞在不同微形态钛合金表面的增殖和贴附状况。结果发现兔腱膜成纤维细胞在光滑组、喷砂组、微沟嵴组、螺纹组表面增殖的光吸收值均数分布有规律,两两比较大都有统计学意义(P<0.05),说明不同的表面微形态对细胞增殖有影响;扫描电镜下观察,光滑对照组表面细胞贴附无明显定向性,不规则的喷砂表面细胞的贴附无规律性,而两种规则微形态表面细胞贴附均按照材料纹理走行的方向贴附,4组样本表面细胞的生长均大致呈长梭形,喷砂表面的细胞表现为部分不规则,细胞间连接丰富,高倍镜下实验组均可见微绒毛清晰的细胞。提示:Ti-Nb-Zr-Sn系钛合金的微形态表面处理比光滑表面更利于动物成纤维细胞的增殖和附着,喷砂表面细胞增殖最快但细胞贴附杂乱,而规则微沟嵴的表面细胞增殖较快且细胞贴附规律,更适合于软组织生理功能的需求。This study was undertaken to assess the effect of different surface microtopograph of Ti-Nb-Zr-Sn al- loy on the biological behavior and soft tissue integration of rabbits＇ lacertus fibroblast. The lacertus fibroblasts of A- chilles tendon of rabbits were cultured and inoculated on the surface of smooth Ti-flake （control group）, and on the surface of sand blast, microgrooves,thread Ti-flakes respectively （3 treatment groups）, The cell＇s growth ratio and attaching status were examined by MTT test and scanning electron microscopy（SEM）. The rabbits~ lacertus fibro- blasts proliferated regularly on the surface of Ti-flakes, and most results of their inter-group comparison showed statistically significant difference （P〈0.05）, thus indicating the different effects of different surface microtopographs on cell^s proliferation; By SEM, the cells were noted to have no obvious directivity on smooth surfacer they attached irregularly on the sand blast surface. But in the other two groups, they were oriented regularly along the texture. The cells were almost shuttle-like on the four kinds of surface except for a part of irregular cells on the sand blast surface, and there were plentiful cell-cell junctions. Under high power lens, we found cells with limpid microvilli in the experiment samples. Owing to the cell＇s fast proliferation and regular attachment in the experiment, we concluded that the regular microgrooved surface was more suitable for meeting the requirement of the soft tissues＇ physiological function.

关 键 词：钛合金表面微形态成纤维细胞 

分 类 号：R318.08[医药卫生—生物医学工程]