地塞米松致C57BL／6J小鼠胚胎腭裂的代谢物组^1H-NMR模式识别探讨  

Investigation on Dexamethasone Induced Cleft Palate in Embryos of C57BL/6J Mice by ^1H-NMR Pattern Recognition of Metabolites Group

作　　者：徐滨[1] 周京琳[1] 何苇[1] 卢胜军[1] 孔祥丽[1] 石冰[1] 黄静[2] 李伟[1] 

机构地区：[1]四川大学口腔疾病研究国家重点实验室,成都610041 [2]四川大学华西药学院,成都610041

出　　处：《生物医学工程学杂志》2009年第2期366-370,共5页Journal of Biomedical Engineering

基　　金：教育部科学技术研究重大项目资助(307022)

摘　　要：代谢组学是在新陈代谢的动态进程中,系统研究代谢产物的变化规律,揭示生命活动代谢本质的科学。本实验制备正常组和实验组各15只怀孕17.5d的C57BL/6J雌鼠(分别于怀孕10～12d腹腔注射生理盐水和地塞米松)的肝组织水溶性提取液,利用核磁共振技术测定其内源性小分子代谢产物,通过主成分分析方法确定代谢物组成分的变化。实验结果表明,两组样本代谢物组在PCA得分散点图上产生了显著的差异,并且与两组胚胎腭裂发生率的差异相符。所以,采用代谢组学的分析方法可以较好的体现C57BL/6J孕鼠在地塞米松干预下妊娠过程中的代谢物组变化,为唇腭裂致病机理的深入研究开辟了一条新的途径。Metabonomics approach is a science that systematically studies the regularity of changes of metabolites and reveals the nature of metabolic activities of the lives in the dynamic process of metabolism. In this study, pregnant C57BL/6J mice were randomly divided into two groups with 15 mice in each. The ones in the experimental group were intraperitoneally injected with Dexamethasone and the others in the control group with isotonic Na chloride from 10th to 12th day of gestational period. On 17.5th day, all the mice were executed. The livers were extracted and prepared into aqueous soluble liver tissue extracts. The technology of nuclear magnetic resonance （NMR） was used to detect the endogenous small molecule metabolites. Finally, through the method of principal component analysis （PCA）, changes of metabolites ingredients could be determined. The experimental results showed that there was significant difference in PCA scores plot between the two groups. Furthermore, the difference was in line with that of incidence of cleft palate in the embryos between the two groups. Therefore, metabonomics results can be used to reflect the changes of metabolites group interfered by Dexamethasone in the course of pregnancy of C57BL/6J mice and this method opens up a new way for further study of pathogenic mechanism of cleft lip with or without palate.

关 键 词：代谢组学 唇腭裂 地塞米松核磁共振C57BL/6J小鼠 

分 类 号：R96[医药卫生—药理学]