缺氧诱导因子-1α在血吸虫病家兔门静脉平滑肌细胞中的表达  

Effect of prostaglandin E1 on expression of hypoxia-inducible factor 1 alpha in rabbits with portal hypertension

作　　者：邹卫龙[1] 陈新国[1] 臧运金[1] 杨镇[2] 

机构地区：[1]武警总医院移植科,北京100039 [2]华中科技大学同济医学院附属同济医院肝脏外科,武汉430030

出　　处：《武警医学》2009年第4期310-312,F0003,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金资助项目(No:30170920)

摘　　要：目的观察缺氧诱导因子(HIF)-1α在血吸虫病家兔门静脉中的表达,探讨其在平滑肌细胞(SMC)活化中的意义。方法血吸虫尾蚴皮肤敷贴法感染家兔构建门静脉高压模型,至150 d分别采用Western blot和免疫组织化学法检测HIF1α和Ⅰ型胶原表达水平。同时,采用光学显微镜和透射电镜比较各组家兔门静脉中膜厚度和SMC超微结构。结果与健康家兔门静脉组织比较,血吸虫病家兔HIF-1α表达显著增高(积分吸光度分别为6.45±2.14和3.27±1.58,P<0.01),Ⅰ型胶原含量显著增加(积分吸光度分别为141.3±28.21和48.53±12.22,P<0.01)。模型组门静脉SMC明显增殖、向合成型成纤维细胞转化,中膜增厚(118.29±36.52)μm。结论血吸虫病家兔门静脉HIF-1α显著增加,可能促进SMC活化,参与门静脉高压血管病变形成。Objective To investigate the expression of hypoxia-inducible factor （ HIF）-1 alpha in rabbits with schistosomiasis and to evaluate its role on activation of portal vein smooth muscle cells. Methods Model rabbits with portal hypertension were used 150 d after infection with cercaria of S. japonicum percutaneously. The expressions of H1F-1α and collagen type Ⅰ were detected by Western bloting and immunohistochemistry staining,respectively. The thickness of tunica media of portal vein and the ultramicrostructure alterations of activated SMC were compared by optical microscopy and transmission electron microscopy among groups. Results Compared with portal vein of healthy rabbits, the expression of HIF-1α augmented markedly （OD： 6.45± 2.14 vs 3.27± 1.58 ,P 〈 0.01 ） , and the contents of collagen Ⅰ increased notablely （OD： 141.3 ±28.21 vs 48.53± 12.22,P 〈0.01 ）. Activation of SMC is a characteristic alteration in portal vein of schistosome hepatic fibrosis. Alterations of this event included transformation to activated myofibroblast-like cells and thickening of tunica media （ 118.29 ±36.52） μm. Conclusions Augmented expression of HIF-1 a is involved in activation of SMC and plays in part, a key role in vasculopatby of portal hypertension.

关 键 词：门静脉高压症/血吸虫性 血管平滑肌细胞 缺氧诱导因子 I型胶原 

分 类 号：R532.21[医药卫生—内科学] R657.34[医药卫生—临床医学]