大豆种传南方菜豆花叶病毒和烟草环斑病毒的GICA-RT-PCR检测  

Detection of southern bean mosaic virus and tobacco ringspot virus in soybean seed by GICA-RT-PCR

作　　者：张晓雷[1] 檀根甲[1] 魏梅生[2] 张永江[2] 李桂芬[2] 

机构地区：[1]安徽农业大学植物保护学院,合肥230036 [2]中国检验检疫科学研究院动植物检疫研究所,北京100029

出　　处：《安徽农业大学学报》2009年第2期319-322,共4页Journal of Anhui Agricultural University

基　　金：国家质量监督检验检疫总局课题(2006IK216)资助

摘　　要：南方菜豆花叶病毒(south bean mosaic virus,SBMV)和烟草环斑病毒(tobacco ringspot virus,TRSV)是我国重要的进境检疫性有害生物,两种病毒均为种传病毒,可随大豆种子实现远距离传播。本研究以这两种病毒的大豆病种子为试材,先用胶体金免疫层析试纸条(GICA)检测,将检测完病毒后的胶体金免疫层析试纸条上的T线和C线剪下,再用RT-PCR的方法将T线和C线上捕获的病毒直接进行扩增,这种将血清学与分子生物学相结合起来所建立的GICA-RT-PCR检测方法,省去了烦琐的总RNA提取的环节,简化了病毒检测的步骤,提高了检测的灵敏度。South bean mosaic virus （SBMV） and tobacco ringspot virus （TRSV） are quarantine pests for China. Both of them can be transmitted through soybean seeds. In this study, gold immunochromatography assay （ GICA） of both viruses was firstly prepared. After the GICA was used to detect the virus from seed, the T and C line of GICA were cut by scissors and applied to RT-PCR analysis. This GICA-RT-PCR method could simplify the routine RT-PCR detection steps, and greatly increased the sensitivity of GICA.

关 键 词：南方菜豆花叶病毒 烟草环斑病毒 胶体金免疫层析 试纸条 RT—PCR GICA-RT—PCR 

分 类 号：S432.41[农业科学—植物病理学]