小鼠卵巢自体移植后血管重建及移植部位血管密度的实验观察  

Experimental Study on the Time of Revascularization Between the Plants of Heterotopical Transplantation and the Part of Displacement and the Density of Graft Parts

作　　者：孙静[1] 党玲[1,2] 王燕蓉[1] 贺晓亮[1] 崔岫[1] 沈新生[1] 朱万平[1] 

机构地区：[1]宁夏医科大学组织学与胚胎学教研室宁夏回族自治区生殖与遗传重点实验室,银川750004 [2]宁夏医科大学护理学院,银川750004

出　　处：《宁夏医科大学学报》2009年第2期141-143,F0002,共4页Journal of Ningxia Medical University

基　　金：国家自然科学基金资助项目(30760264);宁夏科技攻关项目(2007GG068;2007GG071)

摘　　要：目的观察小鼠卵巢自体异位移植物与受体移植部位的血供重建时间及移植部位血管密度对移植物存活的影响。方法成年小鼠卵巢剥膜后一分为二,体外培养3h后进行半卵巢自体异位移植于肾被膜下和颈部皮下,实验分为新鲜组、肾背膜下移植组、颈部皮下移植组,于移植后36 h取材,通过墨汁灌注和组织切片技术,观察卵巢移植后血管重建及卵泡存活情况。结果肾被膜下移植组可见有贯穿肾组织至卵巢组织之间的血管,正常卵泡计数较多,与颈部皮下移植组相比差异具有统计学意义(P<0.05)。肾组织血管密度明显大于颈部皮下组织(P<0.05)。结论富含血管的肾被膜下移植较血管较少的颈部皮下移植更有利于卵巢组织移植后的血供重建。Objective To observe the time of revascularization between the plants of heterotopical transplantation and the part of displacement, and the effect of the density of graft parts on the survival of transplantation. Methods Adult mouse half-ovaries were autotransplanted heterotopically under renal capsule and in neck subcutaneous tissue. The vascular reconstruction and the morphologicle of follicles were observed after transplantation for 36h by Injected with ink suspension and histologic technic. Results It can be seen that the blood vessels throughout the kidney to the graft, there were more normal grafian follicle can be observed than that of the neck subcutaneous tissue group（ P 〈 0.05）. The density of blood vessels of Kidney is greater than the neck subcutaneous tissue （ P 〈 0.05）. Conclusion It is the better transplant parts of the half-ovary heterotopical transplantation for under renal capsule, because there were realized the reconstructed blood supply compared with neck subcutaneous tissue.

关 键 词：半卵巢 自体移植 小鼠 血供建立 

分 类 号：R713[医药卫生—妇产科学]