提高胸腹水肿瘤细胞检出率的方法探讨  

Investigation into Methods to Improvement in Pleuroperitoneal Fluids' Positive Proportion

作　　者：肖同浩[1] 陈寿松[1] 熊丽萍[1] 齐曼丽[1] 陈昕薇[1] 梁励玮[1] 

机构地区：[1]解放军广州军区武汉总医院病理科,湖北武汉430070

出　　处：《临床军医杂志》2009年第2期277-278,共2页Clinical Journal of Medical Officers

摘　　要：目的探讨一种提高胸腹水肿瘤细胞检出率的方法。方法对257例胸腹水标本同时做常规细胞涂片,微量标本涂片,沉渣包埋切片,免疫细胞化学染色。结果常规细胞涂片厚,背景不清晰;微量标本涂片细胞数量少;沉渣包埋切片细胞多而集中,背景清晰,免疫细胞化学染色效果好。结论胸腹水沉渣包埋切片,免疫细胞化学染色,可提高胸腹水肿瘤细胞检出率,对肿瘤诊断和鉴别诊断有重要价值。Objective To investigate how to promote the positive rate of pleuroperitoneal fluid examination. Methods A total of 257 patients with pleuroperitoneal fluids were examined by conventional smearing, micro-quantity specimen smearing, paraffin embedded deposit slicing and immunocytochemical staining. Results Conventional smearing had the disadvantage of cell lapping because the slices were too thick and uneven and micro - quantity specimen smearing had the disadvantage of lack of cells. Paraffin embedded deposit slicing had the advantages of cell quantity abundance and clear background; immunocytochemical staining presented clear staining. Conclusion The positive rate of pleuroperitoneal fluid examination can be promoted with paraffin embedded deposit slicing and immunocytochemical staining, which are helpful to the diagnosis and differential diagnosis of tumor.

关 键 词：胸腹水 细胞病理学 沉渣切片 免疫细胞化学 

分 类 号：R446.8[医药卫生—诊断学]