内外源激素对大花蕙兰类原球茎增殖与分化的影响  

Effects of endogenous and exogenous hormones on multiplication and differentiation of the protocorm like-body of Cymbidium hybridum

作　　者：施隆文[1] 向天勇[1] 傅丹艳[1] 林仲付[1] 

机构地区：[1]嘉兴职业技术学院生物与环境分院,浙江嘉兴314036

出　　处：《浙江农业学报》2009年第2期144-148,共5页Acta Agriculturae Zhejiangensis

基　　金：嘉兴市科技局资助项目(2008AY1002)

摘　　要：将扩繁5代后的大花蕙兰类原球茎置于MS培养基中，以不同浓度水平的IBA，6-BA，活性炭和在缺少6-BA的情况下进行正交试验，观察类原球茎的平均增殖倍数和分化率，以及用高效液相色谱仪测定类原球茎中的6-BA，ABA，LAA，IBA，ZT，KT的含量。结果表明：适合于类原球茎增殖的培养基配方为MS＋IBA 2．0mg／L＋6-BA0．1～3．0mg／L＋活性炭0．5-5．0g／L，适合于类原球茎分化的培养基配方为MS＋IBA 0．1mg／L＋6-BA 1．0mg／L4-活性炭2．0g／L。在多次继代培养的情况下，类原球茎的增殖和分化受到外源激素和内源激素的共同影响，已测到的高含量内源激素ZT，KT部分代替了外源激素6-BA起影响作用。The protocorm like-bodies （PLB） of Cymbidium hybridum after multiplying 5 generations were cultured in the MS medium containing different concentrations of IBA, 6-BA and active carbon, as well as the medium lack of 6-BA. The average multiplying times and differential rates of PLB were measured by the orthogonal test. The contents of 6-BA, ABA, LAA, IBA, ZT and KT in PLB were determined by HPLC. The results showed that the suitable medium formula for multiplying PLB was MS ＋ IBA 2.0 mg/L ＋ 6-BA 0.1 - 3.0 mg/L ＋ active carbon 0.5 - 5.0 g/L and the suitable medium formula for differentiating PLB was MS ＋ IBA 0.1 mg/L ＋ 6-BA 1.0 mg/L ＋ active carbon 2.0 g/L. In the process of multiple subculture, the multiphcation and differentiation of PLB were affected by exogenous hormones and endogenous hormones tngether. For example, the lack of 6-BA in the medium could be made up by the high content of endogenous hormones such as ZT and KT during multiplication and differentiation.

关 键 词：内外源激素 大花蕙兰 类原球茎 增殖与分化 

分 类 号：S682.31[农业科学—观赏园艺]