槲皮素、补骨脂素对人乳腺癌T47D细胞两种受体亚型表达的影响  

Effects of quercetin and psoralen on expression of ERα,ERβ protein levels in T47D cells

作　　者：沈丽霞[1] 牛建昭[2] 王继峰[2] 

机构地区：[1]河北北方学院药理学教研室,河北张家口075000 [2]北京中医药大学细胞生化实验室,北京100029

出　　处：《中国药理学通报》2009年第4期461-464,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30371775;30510403202;30540420032);教育部长江学者和创新团队发展计划资助项目(NoIRT0413);教育部博士点科研基金资助项目(No20050026012)

摘　　要：目的探讨槲皮素和补骨脂素的雌激素作用以及调控雌激素受体亚型表达的作用机制。方法10μmol.L-1槲皮素和10μmol.L-1补骨脂素分别处理T47D细胞48 h,采用RT-PCR和Western blot方法测定对雌激素依赖性乳腺癌细胞T47D雌激素受体亚型ERα和ERβ表达的影响,并以雌激素受体拮抗剂ICI182,780为工具药来评价槲皮素和补骨脂素发挥雌激素样作用与雌激素受体的关系。结果槲皮素、补骨脂素在10μmol.L-1可明显诱导T47D细胞ERαmRNA和蛋白表达,而对ERβ表达没有影响。当与ICI182,780共孵育T47D细胞ERα表达被拮抗。结论槲皮素、补骨脂素产生的促进ER阳性细胞增殖的雌激素样作用是通过增加ERα表达实现的。Aim To investigate the phytoestrogenic effects and mechanism on ERos, ERβ expression levels at the protein of quercetin and psoralen in T47D cells. Methods T47D cells were treated with 10 μmol · L^-1 quercetin and 10 μmol · L^-1 psoralen for 48h respectively, total cell extracts examined for the levels of ERα and ERβ protein by RT-PCR and Western blot analysis. The estrogen-like effect of quercetin and psoralen and their relations with the estrogen-receptor were evaluated by pure estrogen receptor antagonist ICI182, 780 as a tool. Results Psoralen（ 10 μmol · L^-1 ） and quercetin （10 μmol · L^-1） up-regulated the expression of ERα mRNA and protein, without any effect on ERβ mRNA and protein levels. The above up-regulation on ERα protein could be inhibited by adding estrogen receptor antagonism ICI182,780. Conclusion Psoralen and quercetin exerts estrogenic activity to ER- positive cells proliferation through interaction with ERα expression.

关 键 词：植物雌激素 金雀异黄素 槲皮素 补骨脂素 雌激素受体 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]