darbufelone对胃癌SGC-7901细胞增殖、凋亡的影响  

Effect of darbufelone on the proliferation and apoptosis of SGC-7901 cell line

作　　者：潘敏[1] 刘纯伦[1] 方晓杰[1] 石建华[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《中国药理学通报》2009年第4期523-527,共5页Chinese Pharmacological Bulletin

基　　金：重庆市卫生局资助课题(No05-2-155)

摘　　要：目的观察环氧合酶-2/5-脂氧合酶双重抑制剂dar-bufelone对胃癌SGC-7901细胞生长的影响。方法MTT法测定darbufelone对胃癌SGC-7901细胞增殖的影响;TUNEL法检测细胞凋亡;RT-PCR法分析SGC-7901细胞中5-LOX和COX-2 mRNA的表达;免疫细胞化学法检测胃癌SGC-7901细胞中5-LOX和COX-2蛋白质的表达。结果dar-bufelone以时间剂量依赖的方式抑制SGC-7901细胞增殖;1.5×10-5、1.0×10-5和5×10-6mol.L-1darbufelone作用72 h后,细胞凋亡率分别为(30.3±2.1)%、(23.0±2.0)%和(15.0±1.5)%,均高于对照组(0.6±0.1)%(P<0.01);1.5×10-5、1.0×10-5和5×10-6mol.L-1darbufelone处理胃癌SGC-7901细胞72 h后,5-LOX和COX-2 mRNA及其蛋白质表达均减少(P<0.05)。结论环氧合酶-2/5-脂氧合酶双重抑制剂darbufelone对人胃癌SGC-7901细胞株有抑制增殖、诱导凋亡的作用。Aim To investigate the effects of dual COX-2/5-LOX inhibitor darbufelone on the inhibition of gastric adenocarcinoma in vitro. Methods MTT assay was used to detect the inhibition of gastric adenocarcinoma cell line SGC-7901 by darbufelone in different concentrations and at different times. TUNEL staining was used to evaluate the apoptosis of SGC-7901 cells. The changes of 5-LOX and COX-2 mRNA expression were studied by reverse transcription polymerase chain reaction. 5-LOX and COX-2 protein expressions were analyzed by immunocytochemistry in SGC- 7901 cells. Results darbufelone could decrease the proliferation significantly in a dose-dependent and time-dependent manner in SGC-7901 cells. After treating SGC-7901 with darbufelone at concentration of 1.5 × 10^-5,1.0 ×10^-5, 5 ×10^-6mol · L^-1 for72 hs, apoptosis index （% ） of SGC-7901 was （30. 3 ± 2.1）%, （23.0±2.0）%,（15.0 ± 1.5）%, respectively, which was significantly higher than （0.6 ±0. 1 ）% that in control group（P 〈0.01 ）. 1.5× 10^-5, 1.0 × 10^ -5, 5 × 10^ -6 mol · L^-1 darbufelone effectively inhibited the protein and mRNA expression of COX-2 and 5-LOX （ P 〈 0.05 ）. Conclusions darbufelone can inhibit the proliferation and induce apoptosis of SGC-7901 cells.

关 键 词：SGC-7901细胞 环氧合酶-2 5-脂氧合酶 增殖 凋亡 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]