ANGⅡ-AT_1 Receptor Pathway Is Involved in the Anti-fibrotic Effect of β-elemene  

作　　者：朱锐 杨玲 沈霖 叶进 刘建国 胡胜军 

机构地区：[1]Department of Traditional Chinese Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology [2]Department of Gastroenterology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2009年第2期177-181,共5页华中科技大学学报（医学英德文版）

基　　金：supported by a grant from the National Natural Sciences Foundation of China (No.30500658)

摘　　要：To investigate the effects of β-elemene on the ANG Ⅱ-ATI receptor pathway in rats with liver fibrosis, a model of hepatic fibrosis was induced by hypodermical injection of carbon tetrachloride （CC14） into Wistar male rats. D-elemene was intraperitonealy administered into the rats for 8 weeks （0.1 mL/100 g body weight per day）. Masson staining was used to observe the liver fibrosis of rats and liver functions were measured by enzymatic kinetic analysis. The content of hydroxyproline in liver tissues was detected by specimen alkaline hydrolysis. The level of plasma ANG Ⅱ in blood plasma was detected by radioimmunoassay. The expression of AT1R in rat liver were measured using reverse transcriptional-polymerase chain reaction and immunohistochemistry respectively. The results showed that β-elemene could reduce the collagen disposition in liver and inhibit the progression of liver fibrosis. In addition, the levels of plasma ANG Ⅱ and the expression of hepatic AT1R in rats with liver fibrosis were also suppressed by β-elemene. It is concluded that the ANG Ⅱ-AT1 receptor pathway plays an important role in the development of hepatic fibrosis and D-elemene could down-regulate the levels of plasma ANG Ⅱ and the expression of hepatic ATIR in rats with liver fibrosis.To investigate the effects of β-elemene on the ANG Ⅱ-ATI receptor pathway in rats with liver fibrosis, a model of hepatic fibrosis was induced by hypodermical injection of carbon tetrachloride （CC14） into Wistar male rats. D-elemene was intraperitonealy administered into the rats for 8 weeks （0.1 mL/100 g body weight per day）. Masson staining was used to observe the liver fibrosis of rats and liver functions were measured by enzymatic kinetic analysis. The content of hydroxyproline in liver tissues was detected by specimen alkaline hydrolysis. The level of plasma ANG Ⅱ in blood plasma was detected by radioimmunoassay. The expression of AT1R in rat liver were measured using reverse transcriptional-polymerase chain reaction and immunohistochemistry respectively. The results showed that β-elemene could reduce the collagen disposition in liver and inhibit the progression of liver fibrosis. In addition, the levels of plasma ANG Ⅱ and the expression of hepatic AT1R in rats with liver fibrosis were also suppressed by β-elemene. It is concluded that the ANG Ⅱ-AT1 receptor pathway plays an important role in the development of hepatic fibrosis and D-elemene could down-regulate the levels of plasma ANG Ⅱ and the expression of hepatic ATIR in rats with liver fibrosis.

关 键 词：liver fibrosis Β-ELEMENE angiotensin Ⅱ ATI receptors 

分 类 号：R285.5[医药卫生—中药学] R575.2[医药卫生—中医学]