大孔树脂用于雷公藤叶总内酯提取的研究  

Experimental study on the extraction of total triptolides from lactone leaves with macroporous resin

作　　者：王曙东[1] 方李[1] 李汉宝[1] 

机构地区：[1]南京军区南京总医院制剂科,江苏南京210002

出　　处：《医学研究生学报》2009年第4期339-342,共4页Journal of Medical Postgraduates

基　　金：江苏省六大人才高峰基金资助项目(批准号:06-B-028)

摘　　要：目的:雷公藤叶中总内酯的提取方法一般采用水煎氯仿萃取法,因氯仿有毒,对环境有污染,探索用大孔树脂法提取雷公藤叶中的总内酯。方法:采用分光光度法测定雷公藤总内酯,以考察大孔树脂提取的工艺,包括考察水煎液的浓度,醇沉淀醇的浓度及树脂的用量。结果:用水煎醇沉法结合大孔树脂法可富集雷公藤叶中的总内酯,水煎液的药与药液质量之比为1∶2,醇沉淀的醇与药液体积之比为2∶1,树脂用量为15%较好。结论:大孔树脂法可用于雷公藤叶总内酯的提取。Objective： The total triptolides in lactone leaves are generally extracted by decocting with chloroform,which is known to be a poisonous substance and pollutes the environment.The authors aimed to evaluate the use of macroporous resin in the extraction process.Methods： We measured the total triptolides by spectrophotometry and investigated the technology of extracting the total triptolides with macroporous resin,including determination of the concentrations of decoction and alcohol in the alcohol precipitation as well as the consumption of resin.Results： The total triptolides were enriched with the combination method of decoction by alcohol precipitation with macroporous resin.The ratio of concentration medicine to decoction was 1 ∶ 2,and that of the volume proportion of alcohol to decoction in the precipitation of alcohol was 2 ∶ 1.The appropriate amount of resin was 15%.Conclusion： Macroporous resin can be used to extract the total triptolides.

关 键 词：大孔树脂 雷公藤叶 雷公藤总内酯 提取工艺 

分 类 号：R284.2[医药卫生—中药学]