采用高架十字迷宫实验优选抗焦虑复方中药制剂中当归和川芎的制备工艺  

Optimizing preparation technology of Radix Angelicae Sinensis and Rhizome Chuanxiong in anti-anxiety Chinese drugs preparation though using EPM experiment

作　　者：吴立坤[1] 张建军[1] 李伟[1] 袁成[1] 高学敏[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2009年第5期641-643,共3页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:通过小鼠高架十字迷宫药效学实验对抗焦虑复方中药制剂中当归和川芎的两种制备工艺(煎煮法和双提法)进行优选。方法:健康雄性昆明种小鼠,随机分为空白对照组、阳性药地西泮组、抗焦虑中药(归芎煎煮法)高、中、低剂量组、抗焦虑中药(归芎双提法)高、中、低剂量组。空白对照组给予生理盐水及其余各组给药10d,第10d进行高架十字迷宫药效学测试,对开臂时间百分率和开臂次数百分率进行统计。结果:与空白对照组比较,地西泮组和归芎双提法高、中剂量组的开臂时间百分率和开臂次数百分率均有显著性差异(P<0.05),而归芎煎煮法各剂量组未见统计学意义。结论:采用双提法提取当归和川芎制备的抗焦虑中药的抗焦虑药效优于采用煎煮法的制剂。Objective： To optimize two preparation technologies （including decocting method and ambi-extracting method） of Radix Angelicae Sinensis and Rhizome Chuanxiong in anti-anxiety Chinese drugs preparation though using EPM experiment. Methods： KM healthy male mice were randomly divided into control group, diazepam group, anti-anxiety Chinese drugs（using decocting method of Radix Angelicae Sinensis and Rhizome Chuanxiong） high, middle and low doses groups, anti- anxiety Chinese drugs（using ambi-extracting method of Radix Angelicae Sinensis and Rhizome Chuanxiong） high, middle and low doses groups, mice in all groups but the control group were fed with corresponding drugs for 10 days, EPT pharmacodynamics experiments were carried out on the tenth day. Results： Open/Total time percent and Open/Total entries percent of mice in control group were lower than those in anti-anxiety Chinese drugs（using ambi-extracting method of Radix Angelicae Sinensis and Rhizome Chuanxiong） high, middle doses groups, which had obvious difference （ P〈0.05 ）.But no obvious difference was found among all anti-anxiety Chinese drugs（using decocting method of Radix Angelicae Sinensis and Rhizome Chuanxiong） doses groups. Conclusion： The anti-anxiety effect of anti-anxiety Chinese drugs using arnbi-extraeting method of Radix Angelicae Sinensis and Rhizome Chuanxiong surpasses that of using decocting method.

关 键 词：抗焦虑 中药复方制剂 当归和川芎 药效学 优选 制备工艺 

分 类 号：TQ461[化学工程—制药化工]