补阳还五汤抑制脊髓损伤区内硫酸软骨素蛋白聚糖表达的实验研究  

Buyang Huanwu Decoction inhibits the expression of chondroitin sulfate proteoglycans in injuried spinal cord of rat

作　　者：郑格琳[1] 张平[1] 郭洁[1] 刘恩[1] 

机构地区：[1]中国中医科学院望京医院病理科,北京100102

出　　处：《中华中医药杂志》2009年第5期678-680,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局课题(No.04-05JQ06)

摘　　要：目的:研究补阳还五汤对大鼠脊髓损伤区硫酸软骨素蛋白聚糖(CSPGs)表达的抑制作用。方法:利用脊髓半横断损伤大鼠模型,将SD大鼠随机分为正常组、空白对照组和补阳还五汤治疗组、阳性对照组(激素组),每组再随机分为7d、15d、30d等3个时间点,通过免疫组织化学染色检测各组脊髓损伤区硫酸软骨素蛋白聚糖表达的差异;结果:补阳还五汤组(0.85±0.03)与空白对照组(1.62±0.06)相比,脊髓损伤区内硫酸软骨素蛋白聚糖表达受到明显抑制,差异有极显著性差异(P<0.01),激素组(1.76±0.04)与空白对照组(1.62±0.06)相比差异无显著性(P>0.05);结论:补阳还五汤可以抑制脊髓损伤区胶质瘢痕内CSPG的表达,从而抑制CSPG对轴突再生的抑制作用,可能是其促进脊髓损伤后后神经功能恢复的机制之一。Objective： To study whether Buyang Huanwu Decoction can inhibit the expression of chondroitin sulfate proteoglycans in the injuried spinal cord of rat. Methods： SD rats were selected and divided into four groups at random： normal group, control group, Buyan Huanwu Decoction group, methylprednisolone group. The expression of chondroitin sulfate proteoglycans was detected by immunohistochemistry method. Results： The expression of chondroitin sulfate proteoglycans in the injuried spinal cord was apparently inhibited by Buyang Huanwu Decoction （ 0.85±0.03 ）, compared with control group （ 1.62±0.06 ） （P〈0.01）. But the expression of chondroitin sulfate proteoglycans in the injuried spinal cord was not inhibited by methylprednisolone （ 1.76±0.04 ）. Conclusion： Buyang Huanwu Decoction can inhibit the expression of chondroitin sulfate proteoglycans which is the inhibitor of axon regeneration. So this function may be one of the mechanism that Buyang Huanwu Decoction can promote the functional recovery after spinal cord injury.

关 键 词：补阳还五汤 脊髓损伤 硫酸软骨素蛋白聚糖 免疫组织化学 

分 类 号：R285.5[医药卫生—中药学]