耐盐基因Hal1的克隆及表达载体的构建  

Cloning of Hal1 gene and construction of its expression plasmid

作　　者：沈雁[1,2] 陈霞[1] 陈燕[1] 龚毅[1] 陈宇光[2] 

机构地区：[1]中科院上海生命科学研究院,上海200233 [2]上海大学生命科学院

出　　处：《现代农业科技》2009年第7期243-244,共2页Modern Agricultural Science and Technology

摘　　要：以酵母菌株BWG1-7A基因组DNA为模板,利用PCR方法,扩增出耐盐基因Hal1,测序表明该基因全长为885个核苷酸,与已发表的序列NC_001148比较,同源性99.3%。将该基因插入表达载体pYES2的BamHI和EcoRI酶切位点之间,构建表达载体pYES2-Hal1,序列测定完全正确,为植物表达载体的构建打下基础。Taking Saccharomyces cerevisiae BWG1-7A genomic DNA as template ,Hall gene is amplified by PCR.. The amplified product was digested with BamHl and EcoRI enzymes,then ligated into pYES2 vector. The recombinant pYES-Hal1 was successfully constructed and verified by DNA sequence,which provided a foundation of the construction of plant expression vector.

关 键 词：耐盐性 Hal1基因 克隆 

分 类 号：Q785[生物学—分子生物学]