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作 者:朱雅欣[1] 张宏利[1] 李文毅[1] 吴铃[1] 张翠平[1] 龙红梅[1] 王晓[1] 李果[1]
机构地区:[1]上海市内分泌代谢病研究所上海市内分泌代谢病临床医学中心上海交通大学医学院瑞金医院内分泌代谢病科,上海200025
出 处:《上海交通大学学报(医学版)》2009年第4期396-398,403,共4页Journal of Shanghai Jiao tong University:Medical Science
基 金:国家自然科学基金(30771021;30800537)~~
摘 要:目的探讨menin蛋白对SOX4基因表达的调控作用。方法构建包含SOX4启动子的报告基因载体,利用荧光报告基因系统分析menin对SOX4基因启动子活性的影响,运用Real-time PCR检测SOX4基因在MEN1-/-小鼠mef细胞和野生型mef细胞中的表达差异。结果与野生型mef细胞比较,MEN1-/-小鼠mef细胞中SOX4基因启动子活性明显升高;而在MEN1-/-小鼠mef细胞中转入MEN1基因后,SOX4基因启动子活性显著降低。SOX4基因在MEN1-/-小鼠mef细胞中的表达量是在野生型mef细胞中的2.5倍。结论Menin蛋白可抑制SOX4基因表达。Objective To explore the role of menin in regulation of SOX4 gene expression. Methods Reporter gene vectors with SOX4 promoter were constructed, and the influence of menin on SOX4 gene promoter activity was analyzed by dual-luciferase reporter gene assay system. Real-time PCR was employed to detect the expression of SOX4 gene in mef cells of MEN1^-/- mice and wild-type reef cells. Results Compared with wild-type reef cells, the SOX4 gene promoter activity was significantly higher in mef cells of MEN1^-/- mice. After MEN1 gene was transfected into reef cells of MEN1^-/- mice, the SOX4 gene promoter activity significantly descreased. The expression of SOX4 gene in mef cells of MEN1^-/- mice was 2.5 time higher than that in wild-type reef cells. Conclusion Menin can inhibit the expression of SOX4 gene.
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