野大麦耐盐变异体的筛选和植株再生(英文)  

Selection for Sodium Chloride Tolerant Variants of Wild Barley and Plant Regeneration

作　　者：朱进[1,2] 李彦舫 张云 唐家琪[1,2] 杨松涛[1,2] 张亚兰 

机构地区：[1]南京军区军事医学研究所 [2]中国人民解放军农牧大学生物教研室

出　　处：《南京师大学报（自然科学版）》1998年第1期82-85,共4页Journal of Nanjing Normal University(Natural Science Edition)

摘　　要：以野大麦（Ｈｏｒｄｅｕｍｂｒｅｖｉｓｕｂｕｌａｔｕｍ（Ｔｒｉｎ．）Ｌｉｎｋ）的成熟胚为外植体诱导愈伤组织，选取淡黄色、易碎的胚性愈伤组织为材料，进行细胞悬浮培养．当建立起均质、分散且生长迅速的细胞系后，结合诱变剂甲基磺酸乙酯（ＥＭＳ）对细胞的化学处理，在含盐０．８％ＮａＣｌ和１．２％ＮａＣｌ的ＭＳ培养基上筛选耐盐变异体．研究结果表明：筛选得到的变异体能在含盐培养基上持续增殖，而对照组则逐渐褐化死亡．变异体与对照组在游离脯氨酸含量、相对电导率、酯酶同工酶等几个生理生化指标方面存在着较大的差异．耐盐１．２％ＮａＣｌ的愈伤组织的游离脯氨酸含量是对照组的６．６８倍，而相对电导率比对照组小２９．９％，两者的酯酶同工酶酶谱也存在明显的差异．以上分析表明，筛选出的愈伤组织具有一定的耐盐性且较稳定．耐盐愈伤组织转入分化培养基中，可经体细胞胚胎发生和器官分化两种途径再生植株，但分化率相对较低．Mature embryos of wild barley Horedum brevi subulatum (Trin.)Link were used as explants to induce callus.Yellowish and friable embryogenic calli were adopted to establish suspension cell cultures.After suspension cultures treated by ethylmethane sulfonate (EMS), sodium chloride tolerant variants were selected from them which cultured on saline medium.The results obtained from these studies showed that the variants could grow for a long time on 1.2% NaCl containing medium,while the controls died gradually.Free L-proline content of 1.2% NaCl tolerant variants was as much as 6.68 times of that of the control,and relative electrical conductivity ratio was 29.9% less than that of the control.Their esterose isozyme patterns were also different from the control with two additional bands,while their peroxidase isozyme bands were similar.These analyses showed that NaCl-selected calli had the characters of NaCl-tolerance.

关 键 词：野大麦 耐盐变异体 植株再生 筛选 

分 类 号：Q949.714.2[生物学—植物学] S512.303[农业科学—作物学]