植物叶片和冠层光化学反射指数与叶黄素循环的关系  

Relationship between xanthophyll cycle and photochemical reflectance index measured at leaf or canopy level in two field-grown plant species

作　　者：彭涛[1] 姚广[1] 高辉远[1] 李鹏民[1] 王未未[1] 孙山[1,2] 赵世杰[1] 

机构地区：[1]山东农业大学生命科学学院／作物生物学国家重点实验室 [2]山东省果树研究所,山东泰安271018

出　　处：《生态学报》2009年第4期1987-1993,共7页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(30671451,30571125);高校博士点专项基金资助项目(No.20050434007)

摘　　要：以一串红(Salvia splendens Ker-Gawl.)和白车轴草(Trifolium repensLinn.)为材料,使用光谱反射技术测定了这两种植物叶片水平和冠层水平的光化学反射指数(PRI)的日变化,同时使用高效液相色谱分析法测定了这两种植物叶黄素循环的日变化,分析了单叶、群体冠层的PRI与叶黄素循环和叶片实际光化学效率(ΦPSⅡ)和非光化学淬灭(NPQ)之间的关系。结果表明,不论是叶片水平还是冠层水平,两种植物PRI的变化均与叶黄素的脱环氧化程度和NPQ之间呈显著的负相关、与ΦPSⅡ呈显著的正相关。研究结果表明无论叶片水平还是冠层水平上的光谱反射指数均能非常好地反映植物光合机构对光能的利用效率。In order to evaluate the relationship between the photochemical reflectance index（PRI）and xanthophyll cycle,the diurnal variations of spectral reflectance at leaf or canopy level,actual photochemical efficiency of PSⅡ（ΦPSⅡ）,non-photochemical quenching（NPQ）in two field-grown species（Salvia splendens Ker-Gawl.,Trifolium repens Linn.）were analysed,the leaves sampled for reflectance were subsequently harvested to assay xanthophyll cycle pigment content by high-performance liquid chromatography（HPLC）.The results showed that the photochemical reflectance index（PRI）measured at leaf or canopy level was negatively correlated with de-epoxidation status of total xanthophyllpigment pool size（（A＋Z）/（A＋Z＋V））and non-photochemical quenching（NPQ）.The actual photochemical efficiency of PSⅡ（ΦPSⅡ）was positively correlated with the PRI.The results demonstrated that the PRI measured at leaf level or canopy level was a good indicator of light use efficiency in photosynthetic apparatus.

关 键 词：光谱分析仪 光化学反射指数 叶黄素循环 非光化学淬灭 

分 类 号：Q945[生物学—植物学]