棉花MADS-box蛋白基因(GhMADS-13)的克隆和表达分析  

Cloning and Expression Analysis of a MADS-box Protein Gene(GhMADS-13) from Upland Cotton

作　　者：吴东[1] 喻树迅[1] 范术丽[1] 宋美珍[1] 王力娜[1] 

机构地区：[1]中国农业科学院棉花研究所农业部棉花遗传改良重点实验室,湖北安阳455000

出　　处：《基因组学与应用生物学》2009年第2期223-228,共6页Genomics and Applied Biology

基　　金：863计划项目(2006AA10A109);973计划项目(2004CB117306)资助

摘　　要：一组具有MADS-box结构域的转录因子在控制花器官的诱导与发育中起着重要作,以中棉所36(CCRI)均一化全长cDNA文库为基础,结合EST测序分析,分离获得一个棉花的MADS-box基因全长cDNA。该基因cDNA全长1079bp,包含一个732bp的完整的开放阅读框,编码234个氨基酸,具有典型的MADS-box结构域和完整的K区,命名为GhMADS-13(GenBank:FJ409870)。与拟南芥、葡萄等植物的AGL6类基因具有高度的同源性。实时定量RT-PCR分析表明,GhMADS-13在CCRI36的花器官发育中早期表达量逐步增加,后期有下降趋势。推测GhMADS-13可能在开花诱导和花器官发育过程中起着重要作用。A group of regulatory factors containing the MADS-box domain plays an important role in controlling floral organ formation and development. A new cotton flowering-related MADS-box gene cDNA was isolated named as GhMADS-13（GenBank accession number： FJ409870） on this basis of a normalized full-length cDNA library of CCRI 36 combination with the sequencing and analysis of expressed sequence tags （ESTs）. The fragment was 1 079 bp long and contained an open reading flame of 732 bp coding a polypeptide of 234 amino acids. The deduced protein contained conservative MADS domain and semi-conservative K domain. Homology analysis showed that the deduced GhMADS-13 protein was highly homologous to the MADS-box genes AGL6 of Arab idopsis thaliana. Expression of GhMADS-13 was analyzed by the method of real-time PCR. Result showed that GhMADS-13 was more highly expressed in flower buds than apical meristem, and then expressed as a downward tendency after inflorescence, suggesting that the GhMADS-13 might play an important role in the transition from the juvenile to the adult stage.

关 键 词：棉花 MADS-BOX基因 表达分析 

分 类 号：S562[农业科学—作物学] Q943.2[生物学—植物学]