白沙蒿肌动蛋白基因核心片段的克隆和序列分析  

Cloning and Sequence Analysis of Actin Gene Fragment from Artemisia sphaerocephala krasch

作　　者：张一弓[1] 张丽静[1] 刘永红[1] 傅华[1] 

机构地区：[1]农业部草地农业生态系统学重点开放实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《基因组学与应用生物学》2009年第2期251-254,共4页Genomics and Applied Biology

基　　金：国家重点研究发展计划973(2007CB108903);国家自然科学基金(30800801)资助

摘　　要：本研究以荒漠植物白沙蒿总RNA为模板,运用RT-PCR方法扩增出肌动蛋白基因核心序列。将获得的片段克隆到T载体后进行测序,序列分析表明:白沙蒿肌动蛋白基因核心片段长599bp,编码198个氨基酸。将该序列在GenBank中注册,并与多种植物肌动蛋白序列进行同源性比较,发现该片段的核酸序列同源性在75%以上,氨基酸序列同源性在85%以上,具有高度保守性。In this study, total RNA was extracted from A rtemisia sphaerocephala krasch. The A ctin gene fragment was obtained by RT-PCR and was sequenced after cloning into pUCm-T vector. The result revealed that it contains 599 bp, encoding a protein of 198 amino acid. Similarity comparison with other actin gene sequence in the GenBank shows it shares over 75% nucleotide sequence similarity and over 85% amino acid sequence similarity with other actin, which suggests that the A rtemisia sphaerocephala krasch A ctin gene is highly conservative.

关 键 词：白沙蒿 肌动蛋白基因 基因核心片段 序列分析 

分 类 号：Q819[生物学—生物工程]