30周糖尿病模型大鼠胃排空异常及胃黏膜细胞凋亡的实验研究  

Empirical study of gastric emptying and gastric mucosal membrane cell apoptosis about the thirtieth week diabetic model rats

作　　者：陆英杰[1] 连至诚[2] 钟毅 赵自明 

机构地区：[1]佛山科学技术学院动医系,广东佛山528231 [2]广州中医药大学脾胃研究所,广东广州510405 [3]广州市第二中医院,广东广州510400

出　　处：《佛山科学技术学院学报（自然科学版）》2009年第2期68-71,84,共5页Journal of Foshan University(Natural Science Edition)

摘　　要：目的检测30周糖尿病大鼠胃排空及胃黏膜细胞凋亡的情况。方法取SPF级SD大鼠,应用链脲佐菌素(S treptozotoc in,STZ)建立糖尿病大鼠模型,持续30周后,应用酚红实验和光密度的方法检测胃排空,采用T une l法检测胃黏膜的细胞凋亡。结果30周糖尿病大鼠胃排空异常,中药组与模型组比较有所改善;胃黏膜细胞凋亡严重的是模型组,中药组与正常组无显著差异。结论实验30周糖尿病大鼠胃排空异常,胃黏膜有不同程度的细胞凋亡,模型组细胞凋亡程度明显,中药组与正常组无显著差异表明中药对胃黏膜的保护作用。Objective To detect the information of gastric emptying and gastric mucosal membrane cell apoptosis for 30 weeks diabetic rats. Methods To establish 30 weeks diabetic rats model with Streptozotocin （STZ）, then phenolsulfonphthalein test and optical density was used for gastric emptying and Tunel test was detected for gastric mucosal membrane cell apoptosis. Results The test showed the thirtieth week diabetic rats existed abnormal gastric emptying,the Chinese medicine group improved, compared with diabetic model group; the severe gastric mucosal membrane cell apoptosis was diabetic model group, compared with the traditional Chinese medicine group and the controlled group without significant deviation. Conclusions 30 weeks diabetic rats had abnormal gastric emptying, gastric mucosal membrane had different degree cell apoptosis, the apoptotic degree was significant, then the Chinese medicine healing group compared with the controlled group had no significant deviation,thus it showed the Chinese medicine could protect gastric mucosal membrane.

关 键 词：糖尿病鼠 胃排空 胃黏膜 细胞凋亡 

分 类 号：R587.1[医药卫生—内分泌]