Analysis of Bacterial Communities Present in Agaricus bisporus Phase Ⅱ Compost Using Amplified Ribosomal DNA Restriction Analysis(ARDRA)(Ⅱ)——Analysis of Bacterial Communities  

作　　者：HE Lihong YU Rongli CHEN Mingjie PAN Yingjie 

机构地区：[1]Key Laboratory of Applied Mycological Resource and Utilization, Ministry of Agriculture, People＇s Republic of China, Shanghai Research Center for Edible Fungi Biotechnology and Engineering, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China [2]Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China [3]Shanghai Ocean University, Shanghai 200090, China

出　　处：《食用菌学报》2009年第1期6-10,共5页Acta Edulis Fungi

基　　金：Supported by project of China Agricultural Ministry(No.nyhyzx-07-008)

摘　　要：Changes in the bacterial communities inhabiting Agaricus bisporus compost during Phase Ⅱ treatment were monitored using amplified rDNA restriction analysis(ARDRA).Sequence analysis data indicated that the bacterial communities associated with the compost samples were far richer in composition when determined by ARDRA compared with traditional methods based on bacterial isolation.Furthermore,the composition of the communities,especially in terms of the dominant bacteria during each of the four treatment stages(beginning of Phase Ⅱ,after pasteurization,5 days aeration,end of Phase Ⅱ,respectively),were very different.Restriction endonuclease digestion of mainly bacterial clones from four 16S rDNA libraries,C1,C3,C6 and C7,previously constructed on the basis of 16S rDNA fragments amplified from the four different stages of the Phase Ⅱ composting process,revealed the presence of unique microbial types.Thermophilic bacteria belonging to the Bacilli,and previously unreported strains of Trichococcus,Planococcus,Caryophanon,and subclass γ-Proteobacteria,were identified among the clones from the compost sampled during the aeration period(C6).Bacteria belonging to Thermus thermophilus and subclass α-Proteobacteria were detected in C1 and C7 composts sampled at the beginning and the end of Phase Ⅱ,respectively.Clones of some uncultured bacteria were also scored.Changes in the bacterial communities inhabiting Agaricus bisporus compost during Phase Ⅱ treatment were monitored using amplified rDNA restriction analysis （ARDRA）. Sequence analysis data indicated that the bacterial communities associated with the compost samples were far richer in composition when determined by ARDRA compared with traditional methods based on bacterial isolation. Furthermore, the composition of the communities, especially in terms of the dominant bacteria during each of the four treatment stages （beginning of Phase Ⅱ, after pasteurization, 5 days aeration, end of Phase Ⅱ, respectively）, were very different. Restriction endonuclease digestion of mainly bacterial clones from four 16S rDNA libraries, C1, C3, C6 and C7, previously constructed on the basis of 16S rDNA fragments amplified from the four different stages of the Phase Ⅱ composting process, revealed the presence of unique microbial types. Thermophilic bacteria belonging to the Bacilli, and previously unreported strains of Trichococcus, Planococcus, Caryophanon, and subclass γ-Proteobacteria, were identified among the clones from the compost sampled during the aeration period （C6）. Bacteria belonging to Thermus thermophilus and subclass α-Proteobacteria were detected in C1 and C7 composts sampled at the beginning and the end of Phase Ⅱ, respectively. Clones of some uncultured bacteria were also scored.

关 键 词：双孢蘑菇 核糖体 细菌克隆 堆肥 

分 类 号：S646.11[农业科学—蔬菜学] Q522[农业科学—园艺学]