Induction of Bone Matrix Protein Expression by Native Bone Matrix Proteins in C2C12 Culture  

作　　者：ZHEN-MING HU SEAN A. F. PEEL STEPHEN K. C. HO GEORGE K. B. SANDOR AND CAMERON M. L. CLOKIE 

机构地区：[1]Department of Orthopedic Surgery, the First Affiliated Hospital, Chongqing Medical University Chongqing 400016, China [2]Orthobiologics Laboratory, Discipline of Oral and Maxillofacial Surgery Faculty of Dentistry, University of Toronto, Toronto, ON, M5G 1G6 Canada

出　　处：《Biomedical and Environmental Sciences》2009年第2期164-169,共6页生物医学与环境科学（英文版）

基　　金：supported by the Ontario Research and Development Challenge Fund (ORDCF);GenSci Regeneration Sciences Inc. (Toronto,Canada)

摘　　要：Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, and bone sialoprotein （BSP） were detected by immunohistochemistry in C2C12 cultured from day 1 to day 28. Results The signaling of bone matrix protein expression became weaker except for type I collagen, OC and BSP after 5 days. Fourteen days after culture, the positive signaling of type I collagen, OPN, ON, OC, and BSP was gradually declined, and could be detected significantly as compared with that of the negative control on day 28. BMP assay showed that the lkaline phosphatase （ALP） activity was higher in C2C12 culture than in the control during the 14-day culture. Also, total protein and DNA significantly increased during the 14-day culture. High levels of ALP were seen in preosteoblasts and osteoblsts in vivo and in differentiating osteoblasts in vitro. ALP was well recognized as a marker reflecting osteoblastic activity. Conclusion Native bovine BMP induces conversion of myoblasts into osteoblasts, produces type I collagen, and plays significantly role in osteoinduction and bone matrix mineralization of C2C 12 in vitro.Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, and bone sialoprotein （BSP） were detected by immunohistochemistry in C2C12 cultured from day 1 to day 28. Results The signaling of bone matrix protein expression became weaker except for type I collagen, OC and BSP after 5 days. Fourteen days after culture, the positive signaling of type I collagen, OPN, ON, OC, and BSP was gradually declined, and could be detected significantly as compared with that of the negative control on day 28. BMP assay showed that the lkaline phosphatase （ALP） activity was higher in C2C12 culture than in the control during the 14-day culture. Also, total protein and DNA significantly increased during the 14-day culture. High levels of ALP were seen in preosteoblasts and osteoblsts in vivo and in differentiating osteoblasts in vitro. ALP was well recognized as a marker reflecting osteoblastic activity. Conclusion Native bovine BMP induces conversion of myoblasts into osteoblasts, produces type I collagen, and plays significantly role in osteoinduction and bone matrix mineralization of C2C 12 in vitro.

关 键 词：Bone matrix proteins Bone morphogenetic proteins IMMUNOHISTOCHEMISTRY C2C12 

分 类 号：R683[医药卫生—骨科学] R554.105[医药卫生—外科学]