微生物活细胞快速检测的新技术研究  

New technology for rapid detection of microbial viable cells

作　　者：叶若松[1] 万翠香[1] 熊凯华[1] 徐锋[1] 魏华[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌江西330047

出　　处：《中国微生态学杂志》2009年第4期359-361,共3页Chinese Journal of Microecology

摘　　要：微生物活菌分为繁殖能力的活菌和有代谢活动但没有繁殖能力的活菌。传统的平板计数方法无法对有代谢活动但没有繁殖能力的活菌进行准确定量分析。本文综述了微生物活细胞检测新技术的研究进展,包括以PCR为基础的新技术和荧光活化细胞分选术与流式细胞术结合的新技术。Viable cells of microorganisms were classified into cells with or without multiplication ability. Classical counting method was not capable of enumerating the viable cells without multiplication ability and thus could not be used for detection and counting of all viable cells. In this paper the research progresses on the emerging new technology for determination of viable cells were summarized, including reverse transcriptase quantitative PCR, ethidium monoazide PCR,immunocapture universal primers PCR, fluorescence-activated cell sorting and/or flow cytometry.

关 键 词：活细胞 快速检测 

分 类 号：Q93-31[生物学—微生物学]