大黄对脓毒症大鼠血浆高迁移率族蛋白B1表达的影响  

Effect of rhubarb on plasma high mobility group box 1 level in septic rats

作　　者：幸泽茂[1] 卢君强[1] 刘岩松[1] 王义兴[1] 洪全球[1] 龚惠[1] 

机构地区：[1]广东医学院附属深圳市第六人民医院ICU,广东深圳518052

出　　处：《中国急救医学》2009年第4期338-340,共3页Chinese Journal of Critical Care Medicine

基　　金：基金项目：2005-2008年度广东省中医药管理局立项（No.2050052）;深圳市科研基金资助课题

摘　　要：目的观察大黄对脓毒症大鼠血清中高迁移率族蛋白B1（HMGB1）、肿瘤坏死因子-α（TNF—α）的影响，揭示大黄治疗脓毒症的机制。方法采用盲肠结扎穿孔术（CLP）制备脓毒症模型，104只雄性SD大鼠随机分为正常对照组（8只）、CLP组（48只）、大黄治疗组（48只）。分别在术后0、3、8、24、48及72h活杀大鼠留取血标本，采用酶联免疫吸附法测定TNF-α和HMGB-1的含量。结果大黄治疗组血浆TNF-α水平在8h，HMGB1水平在24、48及72h明显低于CLP组，差异有统计学意义。结论大黄通过降低血清中TNF—α和HMGB-1的水平，对脓毒症大鼠具有治疗作用。Objective To observe the influence of rhubarb preparation on high mobility group box 1 protein （ HMGB1 ） and tumor necrosis factor - α（ TNF - α ） in rats with sepsis, and investigate the mechanism of rhubarb on sepsis. Methods A sepsis model reproduced by cecal ligation and puncture （CLP） ,and 104 male SD rats were randomly divided into normal control group（ n = 8） ,CLP group （ n = 48 ） , the rhubarb treatment group （ n = 48 ）. The rats were killed at 0,3,8,24,48 and 72 hours after operation and the Plasma was sampled. The contents of TNF - α and HMGBI were detected by enzyme linked immuneoadsorbent assay. Result TNF - α level of plasma were significant increased at 8h post - CLP compared with rhubarb treatment group. In rhubarb treatment group, HMGB - 1 level decreased in different degrees compared with CLP group at 24,48,72 h. Conclusions Rhubarb possesses the action of treat- ment in rats with sepsis by decreasing the TNF-α and HMGB1 contents.

关 键 词：高迁移率族蛋白B1 大黄 脓毒症 

分 类 号：R285.5[医药卫生—中药学] R631[医药卫生—中医学]