腺苷预处理对缺血／再灌注心肌细胞凋亡和凋亡蛋白Bcl-2／Bax的影响  

Effects of adenosine preconditioning on cardiac myocyte apoptosis and Bcl -2/Bax expression in ischemia/reperfusion rats

作　　者：王荣英[1] 田英平[1] 张晓磊[1] 李旭蕊[1] 石汉文[1] 佟飞[1] 

机构地区：[1]河北医科大学第二医院急诊科,石家庄050000

出　　处：《中国急救医学》2009年第4期340-342,共3页Chinese Journal of Critical Care Medicine

基　　金：基金项目：河北省科技厅科研基金资助项目（No.06276102D-100）

摘　　要：目的研究腺苷预处理对大鼠缺血／再灌注心肌细胞凋亡和凋亡蛋白Bcl-2／Bax的影响。方法制备缺血／再灌注损伤（I／R）和腺苷预处理的大鼠模型，采用流式细胞仪检测心肌细胞凋亡率和凋亡蛋白Bcl-2和Bax。结果①缺血／再灌注组和腺苷组心肌细胞凋亡率均显著高于对照组（P〈0．01），而腺苷组凋亡率显著低于缺血／再灌注组（P〈0．01）。②缺血／再灌注组抗凋亡蛋白Bcl-2表达与对照组比较无明显差别，而腺苷组Bcl-2显著高于缺血／再灌注组和对照组（P〈0．01）；缺血／再灌注组和腺苷组促凋亡蛋白Bax表达显著高于对照组（P〈0．01），而腺苷组Bax低于缺血／再灌注组（P〈0．01）；缺血／再灌注组和腺苷组Bcl-2／Bax比值显著低于对照组（P〈0．01），而腺苷组Bcl-2／Bax比值显著高于缺血／再灌注组（P〈0．01）。结论腺苷预处理明显抑制大鼠缺血／再灌注后心肌细胞的凋亡，并使Bcl-2／Bax比值增加。Objective To investigate the effect of adenosine preconditioning on cardiac myocyte apoptosis and expression of apoptotic protein Bcl - 2/Bax during myocardial ischemia/reperfusion （ I/R ） in rats. Methods A model of I/R injury （ I/R group ） and pretreatment with adenosine （ AP group ） was reproduced in rats. Apoptotic rate of cardiac myocyte and expression of Bcl -2 and Bax proteins in apoptosis myoeardiac cells were observed by flow cytometry （ FCM ）. Result ①Apoptotic rate of cardiac myocyte was high in I/R group and AP group compared with control group （ P 〈 0.01） , but it is lower in AP group than that in I/R group（ P 〈 0.01）. ②There was no difference of the expression of anti -apoptotic protein Bcl- 2 btween I/R group and control group , but but it is higher in AP group than that in I/R group and control group （P 〈0.01 ）. The expression of proapoptotic protein Bax was high in I/R group and AP group compared with control group （P 〈 0.01 ） , but it is lower in AP group than that in L/R group （P 〈 0.01 ）. The Bcl- 2/Bax ratio was significandy lower in I/R group and AP group than that in control group （ P 〈 0.01 ）, but it is higher in AP group than that in I/R group（ P 〈 0.01 ）. Conclusions Adenosine preconditioning could significantly inhibite apoptosis of I/R cardiac myocyte and increase the Bcl - 2/ Bax ratio.

关 键 词：心肌 缺血/再灌注 腺苷预处理 凋亡 大鼠 BCL-2/BAX 

分 类 号：R542.2[医药卫生—心血管疾病]