氧化修饰对转录因子活性的调节  

Oxidative Modification in Regulating the Activity of Transcription Factors

作　　者：桑晶[1] 易静[1] 

机构地区：[1]上海交通大学医学院细胞生物学教研室,上海200025

出　　处：《细胞生物学杂志》2009年第2期191-197,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金项目(No.30570965);上海市科委基础研究重点项目(No.05JC14033);上海市教委重点学科第四期(No.ZDXK2001)资助~~

摘　　要：基因的表达同时受到生理信号和环境刺激的调节。近年来,活性氧被认为是多种生长因子和细胞因子信号通路中的一种第二信使,同时其也产生在细胞损伤应激过程中。活性氧对转录因子的半胱氨酸位点的氧化还原状态有重要的决定作用,其主要影响转录因子二聚体构象的形成、与DNA结合的关键半胱氨酸位点的氧化还原状态、锌指等蛋白质构象的形成。关于氧化还原环境对控制增殖、分化、凋亡功能的转录因子OxyR、Yap-1、AP1、NF-κB、p53、Sp1、USF、HIF-1、C/EBP等的调节,已有大量研究提示氧化还原调节是一种关键的基因表达的调节方式。本文总结了氧化修饰对转录因子发挥调节作用的一般原理和若干典型例子。Gene expression is modulated by both physiological signals and environmental stimuli. Recently, reactive oxygen species （ROS） have been suggested as second messengers for several growth factors and cytokines, and have also been shown to rise following cellular insults. ROS can change the redox states of cysteine residues on transcriptional factors, thus playing an important role in regulating the activity of the transcriptional factors through changing the dimer formation, the DNA binding domain, and the motifs of transactivating domain such as zinc fingers. There are lots of studies about the oxidative modification of transcriptional factors relating with proliferation, differentiation apoptosis, such as OxyR, Yap-1, AP-1, NF-κB, p53, Sp1, USF, HIF-1 and C/EBP, which suggests that oxidative modification is a critical way in regulating gene expression. Here we summarize the rationales and a number of examples for regulating transcription factors by oxidative modification.

关 键 词：活性氧 氧化还原 氧化修饰 转录因子 

分 类 号：Q343[生物学—遗传学]