光肩星天牛幼虫脂肪体原代培养方法的研究  

Evaluation of Some Factors Effecting in Vitro Primary Culture of Larval Fat Body Cells of Anoplophora glabripennis

作　　者：张寰[1] 李瑄[1] 张永安[2] 秦启联[1] 苗麟[1] 王玉珠[2] 曲良建[2] 温发园[2] 张爱君[1] 杨青[1] 

机构地区：[1]中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室,北京100101 [2]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护重点实验室,北京100091

出　　处：《细胞生物学杂志》2009年第2期281-285,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30671688);国家科技支撑计划(No.2006BAD08A1202)资助~~

摘　　要：以光肩星天牛幼虫脂肪体组织为实验材料进行原代细胞培养,研究原代培养过程中,取材的虫龄、脂肪体组织块大小、组织块是否贴壁及培养表面等因素对原代培养及其细胞在体外分离和增殖的影响。结果表明,较高龄的光肩星天牛幼虫(4龄或8龄)的较完整的脂肪体组织,在同培养表面紧密贴附的情况下,原代培养的成功率较高,其细胞在体外从组织块中游离出来并增殖的几率较大。Some factors effecting in vitro primary culture of larval fat body cells of the Asian longhorned beetle （Anopolphora glabripennis） were evaluated in the paper. The results showed that the insect stages, size and integrality of the fat body tissue, character of culture surface and whether the cultured tissues attaching to the culture surface were important for the success of the primary culture. Under the optimized conditions, which were in combination with higher larval stages （4th or 8th instar）, more intact cultured tissue and close attachment of the cultured tissue and the culture surface, the fat body cells were inclined to separate from the cultured tissues and proliferate.

关 键 词：昆虫细胞培养 光肩星天牛 原代培养 细胞增殖 

分 类 号：Q813[生物学—生物工程]