高效液相色谱法测定更昔洛韦凝胶剂中更昔洛韦的含量  

Determination the Content of Ganciclovir in Ganciclovir Gel by HPLC

作　　者：张红[1] 刘祖雄[1] 张孟佑[1] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070

出　　处：《中国药师》2009年第5期606-608,共3页China Pharmacist

摘　　要：目的:建立高效液相色谱法测定更昔洛韦含量的方法。方法:采用Hypersil ODS色谱柱(125mm×4.0mm,5μm);以甲醇-水(7:93)为流动相;流速:1.0ml·min^(-1),柱温:25℃;检测波长:252nm。结果:更昔洛韦的线性范围为2.0～90.0μg·ml^(-1),r=0.999 9。平均回收率为100.0%,RSD为0.5%(n=9)。结论:HPLC法简便,准确,快速,重复性好,可作为更昔洛韦凝胶剂的质量控制。Objective： To establish a method for determination the content of ganciclovir in ganciclovir gel by HPLC. Method： The Hypersil ODS （125mm × 4.0mm,5 μm） column was used, the mobile phase was composed of methanol-water （7：93）, the flow rate was 1.0 ml·min^-1 ; the column temperature was 25℃, and the detection wavelength was 252 nm. Result： The linearity of ganciclovir was good within the range of 2.0 -90.0 μg· ml^-1 （r = 0. 999 9 ）, The average recovery was 100.0%, RSD = 0.5 % （n = 9 ）. Conclusion： This method is simple, accurate, quick and repeatable. It can be used for determination the content of ganciclovir in ganciclovir gel.

关 键 词：更昔洛韦 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]