一种简捷高效提取胚乳细胞RNA的方法  

A High-efficient Protocol to Isolate Total RNA from Endosperm during Middle and Late Development Stage

作　　者：闫洪波[1] 李桂琴[1] 

机构地区：[1]河北经贸大学生物科学与工程学院,石家庄055061

出　　处：《湖北农业科学》2009年第3期522-524,共3页Hubei Agricultural Sciences

基　　金：河北经贸大学博士启动基金[校科字(2005)3号]

摘　　要：在用Trizol一步法提取RNA基础上,通过SDS碱裂解法同Trizol法相结合,得到一种简单、快速从富含多糖及多种次生代谢物的胚乳细胞中提取总RNA的方法。所得RNA样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度、产率和良好的完整性,并可进一步满足RT-PCR和Northern杂交的试验需要。A SDS-Trizol-based extraction method for high-quality total RNA from endosperm cell of wheat and maize was developed. This method could efficiently eliminate the interference of potysaccharide and secondary metabolites in endosperm cell. The results of ultraviolet spectrophotometer and agarose gel electrophoresis analysis show that the obtained RNA has a good purity,high yield rate and no obvious degradation sufficient for further RT-PCR and RNA gel blotting.

关 键 词：胚乳细胞 SDS法 基因扩增 

分 类 号：Q789[生物学—分子生物学]