5ml大管低温冷冻对猪精子顶体和质膜超微结构的影响  

Effects of 5 ml Straws Cryopreservation on the Quality and Ultrastructure of Boar Semen

作　　者：殷方芝[1,2,3] 戴建军[1,2] 吴华莉[1,2] 吴彩凤[1,2] 张廷宇[1,2] 王磊[1,2] 杨山亭[1,2] 张德福[1,2] 

机构地区：[1]上海农业科学院畜牧兽医研究所,上海201106 [2]上海农业遗传育种重点实验室,上海201106 [3]南京农业大学动物科技学院,江苏南京210095

出　　处：《中华男科学杂志》2009年第4期322-325,共4页National Journal of Andrology

基　　金：上海市科技兴农重点推广应用项目;(沪农科推字(2007)第3-7号)、(沪农科攻字(2003)第14-1号)~~

摘　　要：目的:探讨猪精液5ml大管冷冻法与颗粒法、0.25ml细管法冷冻后效果是否存在差异,以及5ml大管冷冻后猪精子的超微结构变化。方法:比较5ml大管、颗粒和0.25ml细管3种剂型冷冻猪精液的效果,利用透射电镜技术,观察5ml大管精液超低温冷冻前后猪精子的形态和超微结构。结果:5ml大管冷冻后精子活动率、存活率和顶体完整率与其它两种剂型差异不显著;5ml大管法冷冻后精子的顶体膜和质膜肿胀,大都呈泡状化,部分局部断裂。结论:猪精液5ml冷冻效果接近0.25ml细管和颗粒法冷冻效果,该冷冻方法仍对猪精子品质和超微结构造成一定的损伤。Objective ： To investigate damages to the quality of boar sperm frozen in 5 ml straws, pellet and 0.25 ml straws as well as the ultrastructural changes of frozen boar sperm in 5 ml straws. Methods ： We compared 3 different freezing packages of 5 ml straws, pellet and 0.25 ml straws to determine their effects on frozen boar semen, and observed the morphological and uhrastructural changes of the boar sperm in the 5 ml straws using scanning electron microscopy. Results ： In the 5 ml straws, the vitality and motility of the boar sperm after freezing were not significantly different from those in the other two formulations, the normal apical ridge （NAR） was 52.65%, higher than in the pellet but not significantly different from that in the 0.25 ml straws, and the sperm membranes were mostly bubbly, some locally broken, which indicated the damage induced by freezing and thawing. Conclusion： At the present time, boar semen frozen in 5 ml straws were not significantly different from those frozen in 0.25 ml straws. The existing freezing-thawing method may cause certain damage to the quality and ultrastructure of boar sperm, and therefore needs to be further improved.

关 键 词：猪 精液 大管 超微结构 低温冷冻 

分 类 号：S828[农业科学—畜牧学]