红景天苷对负性心理应激下模拟航渡并高强度运动雄性大鼠下丘脑-垂体-性腺轴的调节  

Salidroside Protects the Hypothalamic-Pituitary-Gonad Axis of Male Rats Undergoing Negative Psychological Stress in Experimental Navigation and Intensive Exercise

作　　者：王群[1] 王坚[1] 孙玲君[1] 胡兰萍[1] 李洁[1] 邵加庆[1] 卢斌[1] 王扬天[1] 吴波[1] 王国洪[1] 

机构地区：[1]南京大学医学院临床学院/南京军区南京总医院内分泌科,江苏南京210002

出　　处：《中华男科学杂志》2009年第4期331-336,共6页National Journal of Andrology

基　　金：南京军区"十一五"面上课题(06MA132)~~

摘　　要：目的:通过红景天苷对模拟航渡并高强度运动大鼠的干预,观察干预后大鼠下丘脑-垂体-性腺(HPG)轴的功能及超微病理学的改变。方法:将6周龄的SD雄性大鼠随机分为非应激对照组(NS组,n=10)、训练对照组(TC组,n=12)和红景天苷干预组(ST组,n=12)。NS组不接受任何刺激,在生理盐水灌胃1周后即进行取样。TC组和ST组大鼠经过10d递增负荷跑台训练,分别用生理盐水和红景天苷灌胃1周后,进行模拟航渡及高强度运动,结束即刻取血,使用放免法测定大鼠血清睾酮(T)、皮质酮(CORT)、促肾上腺皮质激素(ACTH)、促黄体生成素(LH)和促性腺激素释放激素(GnRH);HE染色观察睾丸组织病理结构;电镜下观察垂体、睾丸超微结构。结果:①TC组大鼠与NS组相比,血清T水平显著下降;灌服红景天苷后与TC组相比,血清T水平显著增高,与NS组比较差异无统计学意义;②HE染色示3组大鼠的睾丸组织病理无明显变化;③电镜检查示TC组垂体细胞胞浆中分泌颗粒显著减少,而ST组与TC组相比胞浆中分泌颗粒的数目增多明显;TC组睾丸Leydig细胞线粒体肿胀,电子密度增高,嵴减少或消失,ST组睾丸Leydig细胞中见线粒体结构趋向于正常,嵴大多数清晰,可见大小不等深染的分泌颗粒。结论:负性心理应激及高强度的运动训练会抑制HPG轴的功能,灌服红景天苷对HPG轴具有保护作用。Objective ： To study the effects of salidroside on the function and ultramicro-pathological change of the hypothalamic-pituitary-gonadal （HPG） axis of male rats in experimental navigation and intensive exercise. Methods： Six-week SD rats were randomized into 3 groups： non-stress control （NC, n = 10）, training control （TC, n = 12） and salidroside treatment （ST, n = 12） group. Blood samples were collected from the NC rats that did not receive any stimulus after a 7-day intragastric administration of saline. The TC rats underwent a 10-day running training with increasing load on the treadmill followed by a 7-day intragastric administration of saline. The ST rats were subjected to the same process of running training as the TC group and received intragastric administration of salidroside. Then blood samples were immediately obtained and the levels of testosterone （T） , corticosterone （CORT）, adrenocorticotropic hormone （ACTH） , luteinizing hormone （LH） and gonadotropin-releasing hormone （GnRH） measured by radioimmunoassay. The testis histopathology was observed by HE staining, and the ultrastructural changes of the pituitaries and testes investigated by electron microscopy. Results： The serum T level was significantly lower in the TC than in the NC group, but showed no significant difference between the ST and NC groups. HE staining revealed no significant difference in testis histopathology among the 3 groups. Ultramicro-pathology showed that the secretory granules of the pituitary cells were significantly reduced in the TC rats compared with the NC ones; the number of the granules significantly increased in the ST group compared with the TC rats; and mitochondrial swelling, increase of electron density and decrease/disappearance of mitochondrial cristae were observed in the Leydig cells of the TC rats. But no significant differences were found in the testicular cells between the ST and NC groups. Conclusion ： Negative psychological stress and intensive exercise can sign

关 键 词：模拟航渡 高强度运动 红景天苷 下丘脑-垂体-性腺轴 睾酮 

分 类 号：R285.5[医药卫生—中药学]