柿果实多聚半乳糖醛酸酶基因克隆与序列分析  

Cloning and Sequencing Full-length cDNA Encoding PG Gene from Persimmon

作　　者：刘乐[1] 饶景萍[1] 常晓晓[1] 

机构地区：[1]西北农林科技大学园艺学院农业部西北园艺种质资源与遗传改良重点开放实验室,陕西杨陵712100

出　　处：《西北植物学报》2009年第4期656-661,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30771756)

摘　　要：以‘富平尖柿’（Diospyros kaki L．cv．FupingJianshi）为材料，采用RACE方法，首次获得了柿果实多聚半乳糖醛酶（PG）基因的3个全长cDNA（登录号为EU816197、EU816198、EU816199），分别命名为DKPG1、DKPG2、DKPG3。DKPG1全长1616bp，DKPG2全长1654bp，DKPG3全长1545bp。3个基因均含有一个1326bp的开放阅读框，共编码441个氨基酸。通过Blast比对发现该基因核苷酸序列与其他植物已报道的PG基因具有74％～78％的相似性；其氨基酸序列与其他植物的相似性为60％～73％。对GenBank同源性搜索获得的其他植物PG基因氨基酸序列进行系统进化分析，发现其与葡萄、猕猴桃、桃的亲缘关系近，与大豆亲缘关系较远。Persimmon （Diospyros kaki L. ） ‘Fuping Jianshi’ was used in the present study. Three full- length PG cDNA were generated via DNASTAR by Splicing the sequences obtained from 3＇-RACE and 5＇- RACE technology. （GenBank accession number ：EU816197,EUS16198,EU816199）. DKPG1 was 1 616 bp in length,DKPG2 was 1 654 bp in length,DKPG3 was 1 545 bp in length. They are all coding 441 amino acid. The results of homologous analysis in GenBank demonstrated that the sequences had 74%～78% identity on the nucleotide sequence and 60%～73% identity on the deduced amino acid sequence. The results of phylogenetie analysis showed that PG gene from persimmon and from Actinidia chinensia ,Vitis vinifera and Amygdalus persica clustered together,and then came those from Glycine max.

关 键 词：PG酶 CDNA克隆 柿 

分 类 号：Q789[生物学—分子生物学]