采用酵母双杂交系统筛选GmDREB5的互作蛋白  

Screening of Proteins Involved in the Interaction with GmDREB5 Using Yeast Two-Hybrid System

作　　者：曹新有[1,2] 刘阳娜[1,2] 陈明[2] 李连城[2] 陈耀锋[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100 [2]中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,农业部作物遗传育种重点开放实验室,北京100081

出　　处：《西北植物学报》2009年第4期662-668,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家"863"项目(2006AA10A111);国家自然科学基金项目(30700508)

摘　　要：以无自激活活性的GmDREB5蛋白73～226位氨基酸区段为诱饵，采用酵母双杂交系统筛选干旱处理5h大豆cDNA文库。结果发现：一个互作蛋白含有保守的TPR（Tetratricopeptide repeat）结构域，与拟南芥的TPR蛋白仅有14％的相似性，说明其可能是一类新的大豆TPR蛋白，将其定名为GmTPR1；表达特性分析表明，GmTPR1基因受干旱、低温、高盐、ABA的诱导；证明GmTPR1不仅参与植物对非生物胁迫的响应，同时参与对GmDREB5蛋白水平的调控。The interacting proteins of GmDREB5 was attained by screening the cDNA library of soybean under drought and identified by yeast-two hybrid system,which used the segment containing 73～226 amino acid of GmDREB5 as bait. It was found that there was a conservative domain TPR （Tetratricopeptide repeat） in the interacting proteins, and which shared 14% similarity of the amino acid sequence with TPR protein in Arabidopsis thaliana. The interacting protein with GmDREBS, named GmTPR1, was isolated and proven to be a TPR protein in soyb）an. Further analyses showed that the expression of GmTPR1 gene in soybean was induced by drought, cold stresses,high concentration salt and ABA treatment. These results suggested that GmTPR1 might involve in not only response to abiotic stress but also regulation of Gm-DREB5 by interacting in soybean.

关 键 词：大豆 DREB转录因子 酵母双杂交系统 互作蛋白 

分 类 号：Q789[生物学—分子生物学]