NaCl胁迫对盐芥质膜和液泡膜ATPase活性的影响  

Changes of PM and TP ATPase Activities in Thellungiella Seedlings under Salt Stress

作　　者：赵昕[1] 谭会娟[1] 丁楠[2] 

机构地区：[1]中国科学院寒区旱区环境与工程研究所极端环境生物抗逆特性实验室,兰州730000 [2]兰州大学生命科学学院,兰州730000

出　　处：《西北植物学报》2009年第4期717-723,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30770343);中国科学院寒区旱区环境与工程研究所科研基金

摘　　要：以盐生植物盐芥和中生植物拟南芥幼苗为材料，研究了盐胁迫对它们叶片和根质膜、液泡膜H^＋-ATPase、Ca^2＋-ATPases和K^＋-ATPase活性以及H^＋-ATPase、Na^＋／H^＋逆向转运蛋白表达的影响。结果显示：在NaCl胁迫下，盐芥叶片和根质膜的H^＋-ATPase活性分别比对照显著升高41％～212％和35％～53％，液泡膜的HtATPase分别显著升高281％～373％和4％～38％，而拟南芥却比相应对照都显著降低；相同盐浓度胁迫下，盐芥叶片的H^＋-ATPase活性比根部高4～8倍，盐芥根也远高于拟南芥。在NaCl胁迫下，盐芥叶片和根的液泡膜HtATPase蛋白质B亚基含量变化与其酶活性变化趋势一致，质膜Na^＋／H^＋逆向转运蛋白的表达量与Na^＋含量变化趋势一致。盐胁迫下盐芥根中Ca^2＋-ATPases和KtATPase活性的增加与根中Ca^2＋和K^＋含量呈显著正相关。研究发现，在盐胁迫条件下，盐芥能有效增强H^＋-ATPase蛋白和Na^＋／H^＋逆向转运蛋白表达，显著提高其根系与叶片质膜和液泡膜的H^＋ATPase、Ca^＋ATPase和K-1ATPase活性，维持细胞质中较高的Ca^＋和K^＋水平，从而缓解盐胁迫的伤害，增强耐盐性。The effect of salt stress on the modification of plasma membrane （PM） and tonoplast （TIP） H^＋- ATPase activities in Thellungiella （Thellungiella halophila） and Arabidopsis seedlings were studied. Under 100-400 mmol·L^-1 NaCl stress, the H^＋-ATPase,Ca^2＋-ATPase and K^＋-ATPase activities in Thellungiella roots and leaves increased about 41%-212% ,35%-53% in PM and 281%-373%,4%-38% in TP than the untreated plant. Under the same NaC1 concentration stress,the H^＋-ATPase,Ca^2＋-ATPase and K^＋-ATPase activities in Thellungiella leaves were 4～8 times than those in its roots. The TP H^＋-AT- Pase 13 subunit Western blotting results showed the same increased level as the H^＋-ATPase activity in roots,and PM Na/I-t antiporter Western blotting results showed significant difference with the different Na＋ contents in its leaves and roots. Ca^2＋-ATpase and K^＋-ATpase activities were highly correlated with Ca^2＋ and K^＋ concentration in Thellungiella roots treated by NaCl. It suggested that Ca^2＋ was the second signal transduction in Thellungiella root and involved in feedback regulation of PM H^＋-ATPase. These results indicated that Thellungiella enhanced the salt resistant characteristic through increasing the roots and leaves H^＋ ATPase,Ca^2＋ ATPase and K^＋-ATPase activities.

关 键 词：盐胁迫 盐芥 质膜 液泡膜 H+-ATPase 

分 类 号：Q945.78[生物学—植物学]