EPO微球剂型在降低小鼠免疫原性方面的作用  

Effect of EPO Microspheres on Reducing Immunogenicity in Mice

作　　者：郭梅燕[1] 赵昊[1] 陈景乐[1] 任甜甜[1] 袁伟恩[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《现代生物医学进展》2009年第8期1419-1420,1404,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30873180)

摘　　要：目的:观察EPO微球剂降低小鼠免疫原性方面的优势。方法:W0W复乳法制备EPO微球,对BALB/C小鼠进行皮下注射,并利用水针剂型作为对照,采用促UT-7细胞生长抑制法检测小鼠体内的EPO抗体,观察小鼠血清中EPO抗体的量。结果:水针组小鼠血清加入细胞培养液以及一定效价的EPO溶液培养染色裂解后,吸光值比空白值有较大降低,说明其中的EPO抗体量较多。而W/O/W微球组小鼠血清中的抗体则比水针组要少,这说明EPO做成微球剂型,给药后可以减少抗体的产生,在降低免疫原性方面优于水针组。结论:微球剂型给药比水针在降低免疫原性方面具有优势。Objective： To investigate the effect of EPO microspheres on reducing immunogenicity in the mice. Methods： Prepare EPO microspheres by WOW methods and select the EPO solution as control. Administer the microspheres and solutions through subcutaneous route. Then, detect anti-EPO antibodies developed in the mice through UT-7 cells proliferation assays. Results： Compared with those given EPO solutions, the experiment results indicated a reduced antibody-development in the mice which were given microspheres by W/O/W. Conclusion： EPO microspheres have an advantage in reducing immunogenicity of EPO in the mice, compared to EPO solutions.

关 键 词：EPO 抗体 MTT法 细胞生长抑制法 

分 类 号：Q95-3[生物学—动物学] Q78