水稻CesA4基因启动子与GUS基因融合表达  

Localization of Rice CesA4 Promoter with GUS-Fusion

作　　者：王文芳[1,2] 刘爱玲[1,2] 李琦[1] 邹杰[1,2] 周小云[2] 许发喜[1,2] 李尉[1,2] 王育华[1,2] 陈信波[1,2] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南农业大学国家重点实验室培育基地作物基因工程实验室,湖南长沙410128

出　　处：《现代生物医学进展》2009年第8期1459-1461,1554,共4页Progress in Modern Biomedicine

基　　金：国家973计划前期研究专项(2007CB116207);湖南省科技厅重点实验室重点项目(06FJ3002);教育部博士点基金(20050537001)

摘　　要：目的:利用GUS报告基因,研究水稻CesA4基因在水稻组织和器官的定位。方法:克隆水稻的CesA4基因的启动子并用GUS组织化学染色检测启动子与GUS基因融合表达情况。结果:成功克隆了水稻的CesA4基因的启动子,并发现水稻的CesA4基因在水稻的根、茎、叶、鞘、穗的纤维均有表达。结论:通过将水稻CesA4基因的启动子与GUS基因融合来分析水稻此基因的表达部位,是一种简便和有效方法。Objective： To study the localization of rice CesA4 gene in rice tissues and organs with GUS reporter gene. Methods： The rice CesA4 promoter was cloned. The expressions of the rice CesA4 gene promoters and the GUS gene were detected by GUS staining. Results： The cloning office CesA4 promoter gene was successful. It was found that the rice CesA4 gene expressed in rice fibers of roots, stems, leaves, sheath and spike. Conclusion： It is a simple and effective way to locate the rice CesA4 gene expression in rice by fusing the gene promoter and the GUS gene.

关 键 词：水稻 CesA4基因 启动子 GUS 

分 类 号：Q943.2[生物学—植物学] S511.22[农业科学—作物学]