不同处理对山羊精子转染外源DNA效率的影响  

The Effect of Gene Transfection of Foreign DNA into Goat Sperm by Different Treatments

作　　者：范景胜[1,2] 赵永聚[1,2] 徐恢仲[1,2] 王岭斌[1,2] 于明举[1,2] 

机构地区：[1]西南大学动物科技学院,重庆400715 [2]西南大学重庆市牧草与草食家畜重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2009年第4期126-130,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30600430)

摘　　要：将采集的山羊精液去除精清,分为鲜精组、冻精组、反复冻融致死组3组进行处理后,与地高辛标记的线性化PCS2EGFP质粒共育转染,用免疫组化的方法检测精子转染外源DNA的效率.结果显示鲜精、冻精和反复冻融致死3种处理组的阳性转染效率分别为(36.61±7.91)%,(32.68±3.38)%,(69.36±4.79)%.反复冻融致死组转染率极显著高于鲜精组和冻精组(p<0.01),鲜精组和冻精组转染效率差异不显著(p>0.05).Goat semen was centrifuged and divided into three groups：fresh sperm, frozen-thawed sperm and dead sperm by repeated frozen-thawed treatment. The sperms were incubated in vitro with foreign DIG-labeled linear PCS2EGFP plasmid. The transfection rates of the sperms were determined immunohistochemically. The results showed that the sperm DNA-binding rates were （36.61±7.91）%, （32.68±3.38）% and （69.36±4.79）%, respectively, for the above-mentioned three groups, and the sperm DNA-binding rate of dead sperms with repeated frozen-thawed treatment was significantly higher than that of the other two groups （p〈0.01）.

关 键 词：山羊精子 外源DNA 转染 

分 类 号：S827[农业科学—畜牧学]