缺血预适应对大鼠肢体缺血再灌注后肺损伤时黏附分子表达的影响  

Effect of ischemia preconditioning on the expression of adhesion molecules in lung injury following ischemia-reperfusion of extremities in rats

作　　者：徐亚平[1] 徐梅[1] 高福云[1] 赵利军[2] 刘秀德[2] 门秀丽[2] 

机构地区：[1]中日友好临床医学研究所,北京100029 [2]华北煤炭医学院病理生理教研室

出　　处：《解放军医学杂志》2009年第5期569-571,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨缺血预适应对大鼠肢体缺血再灌注致肺损伤时黏附分子ICAM-1表达的影响。方法雄性Wistar大鼠30只,随机分为对照组、缺血再灌注(IR)组和缺血预处理(IPC+IR)组,每组10只。采用流式细胞技术、反转录聚合酶链反应(RT-PCR)和Western blotting等观察肢体缺血再灌注后肺损伤发生过程中,血液中CD18阳性的多形核白细胞(PMN)百分率,以及肺组织ICAM-1在mRNA和蛋白水平的变化;测定肺组织湿/干重值(W/D);检测肺组织中髓过氧化物酶(MPO)含量;观察肺组织形态学的改变及缺血预适应对上述指标的影响。结果大鼠肢体缺血再灌注后W/D及CD18阳性细胞数均明显增加,肺组织MPO活性增加,ICAM-1表达明显升高,预适应可以明显抑制这些变化,从而对肺起一定保护作用。结论缺血预适应对肢体缺血再灌注后肺损伤的保护作用与下调黏附分子表达有关。Objective To investigate the effect of ischemia preconditioning （IPC） on the expression of ICAM-1 in lung injury after ischemia reperfusion of extremities （LIR） in rats. Methods 30 male healthy Wistar rats were randomly divided into three groups （10 each） ： Control group, ischemia-reperfusion group （IR） and ischemia preconditioning group （IPC）. The positive rate of CD18 in blood polymorphonuclear neutrophil （PMN） was measured by using flow cytometry. The expression of intercellular adhesion molecule-1 （ICAM-1） in lung tissues was detected by reverse-transcription polymerase chain reaction （RT-PCR） and Westem blotting. The levels of wet/dry ratio （W/D） of lungs of different groups were determined. The biochemical method was used to measure the contents of myeloperoxidase （MPO） in lung tissues of different groups. The pathological changes in lungs were observed with light microscope. Results It was found that the levels of MPO and W/D were increased and the expression of ICAM1 was up-regulated in lungs of IR group after rats＇ limbs suffering from ischemia-reperfusion （P〈0. 01 or P〈0. 05）. However, ischemia preconditioning significantly decreased fluorescent positive rate of PMN CD18, attenuated the increase of MPO and W/D of lungs. The results of RT-PCR and Western blotting showed that the expression of ICAM-1 was down-regulated in lung tissue of IPC group than that of IR group. Pulmonary pathology revealed congestion and swelling in pulmonary microvessels with broadened pulmonary interstitial tissue and leucocyte infiltration dominated by neutrophils after limbs＇ IR. Conclusions Ischemia preconditioning may protect lung tissues from limb ischemia-reperfusion injury in rats, which is related to the down regulation of ICAM-1 expression.

关 键 词：肺损伤 再灌注损伤 胞间黏附分子1 

分 类 号：R563.9[医药卫生—呼吸系统]