热应激小鼠附睾组织HSP70表达的研究  

Study of HSP70 Expression in Heat Stress Mice Epididymis

作　　者：姜忠玲[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109

出　　处：《中国农学通报》2009年第8期12-15,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然基金项目"奶牛胚胎热休克蛋白70对胚胎抗热作用的研究"(30571338)

摘　　要：利用化学恒温培养箱对小鼠实施42℃ 1h/天的热应激处理,建立持续的全身热应激动物模型。在热应激持续到8、13、21和35天时,颈椎脱臼处死对照组和热应激组小鼠,应用改良巴氏染色法检测附睾精子畸形率,免疫荧光组织化学和蛋白质印迹分析的方法检测附睾组织HSP70的表达。结果显示:热应激组小鼠附睾精子畸形率随着热应激持续时间的延长而升高,且显著(P<0.05)高于对照组;对照组小鼠附睾组织HSP70呈极强表达(+++),而热应激组小鼠附睾组织HSP70的表达随着热应激持续时间的延长而减弱,当热应激持续到35天时呈极弱的表达(±)。结果表明:热应激损伤了附睾的功能,使附睾精子畸形率增加;附睾内HSP70为非热诱导型,HSP70可能在附睾精子的成熟过程发挥特殊的作用。Mice were treated by heat stress at the temperature 42 ℃ and one hour per day using incubator, and the sustaining systemic heat stressed animal model were established by this way. The control group and heat stress group mice were killed by cervical dislocation, when they were treated duration of heat stress to the day 8, 13, 21 and 35, and the percentage of abnormal sperm in the epididymis was detected by using the modified Papanicolaou staining method, and the expression of HSPT0 in the epididymis detected by immunofluoreseence histochemistry and western blot analysis methods. The result showed： the percentage of abnormal sperm in the epididymis of heat stress group was increased with the prolongation of heat stress time, and was obviously higher than the control group; There was a strong expression of HSP70 in the control group（＋＋＋）, however, the expression of HSPT0 in the epididymis was weakened with the prolongation of heat stress time, 35d after the heat stress there was a very weak expression（＋）. The result indicated： The function of epididymis were damaged by heat stress, and the sperm deformity rate in the mice epididymis was increased; HSP70 in the epididymis was non heat inducible, it may played special effect in maturation process of epididymis sperm.

关 键 词：热应激 热休克蛋白70 免疫荧光组织化学 蛋白质免疫印迹 附睾 

分 类 号：S852.3[农业科学—基础兽医学]