木槿花粉活力检测方法筛选  

Selection of Pollen Viability Test Way of Hibiscus syriacus

作　　者：张辛华[1,2] 李秀芬[2] 张德顺[3] 刘庆华[1] 杜纪红[2] 王翔[1] 

机构地区：[1]青岛农业大学园林园艺学院,山东青岛266109 [2]上海农科院林木果树研究所,上海201106 [3]同济大学建筑与城市规划学院,上海200292

出　　处：《中国农学通报》2009年第8期227-229,共3页Chinese Agricultural Science Bulletin

基　　金：上海市农委科技攻关资助项目"木槿属观赏树种种质资源收集创新及定向培育技术研究"[沪农科攻字(2005)第l-l-7号]

摘　　要：以2个木槿(Hibiscus syriacus)品种花粉为材料,用TTC法和离体萌发法对木槿花粉活力进行了检测。结果表明:木槿花粉活力检测不适合用TTC法;以木槿花粉为外植体,采用蔗糖悬浮溶液为培养基,确定适合木槿花粉萌发的条件为15%蔗糖溶液+15mg/L硼酸在30℃恒温培养箱中培育0.5h即开始开始萌发。The pollens of 2 cultivators from Hibiscus syriacus were studied. The pollen viability were tested by means of TTC and germination in vitro. The results showed that only when in vitro medium, the pollen germination rate could be improved and represent the pollen viability. The pollen was used as explant and the sucrose solution as medium. The germination of the pollen medium of Hibiscus syriacus was observed, The pollen began germinating with 15% sucrose concentration and 15mg/L boric acid under 30℃ after 30 minetes.

关 键 词：木槿 花粉 TTC 培养基 

分 类 号：S3[农业科学—农艺学]