海南两个自然保护区野生荔枝遗传多样性研究  

Studies of Allozyme Heredity Diversity of Wild Litchi in Two Hainan Nature Protection Areas

作　　者：姜成东[1] 蔡胜忠[2] 肖翔 肖小军[1] 卢业凌[1] 

机构地区：[1]海南大学园艺园林学院,海南儋州571737 [2]中国热带农业科学院橡胶研究所,海南儋州571737 [3]贵州省遵义市正安县农业局,贵州正安563403

出　　处：《中国农学通报》2009年第9期282-286,共5页Chinese Agricultural Science Bulletin

基　　金：海南省自然科学基金"海南野生荔枝分子水平遗传多样性及保育研究"(80663);海南大学自然科学基金"野生荔枝分子水平遗传多样性研究"(Rnd0414)

摘　　要：采用常规聚丙烯酰胺凝胶电泳技术,对海南吊罗山和霸王岭两个国家级自然保护区野生荔枝等位酶遗传多样性进行了研究。13个酶系统、18个酶位点的检测结果表明,两个自然保护区野生荔枝具有较低的居群间遗传变异水平和较高的居群内遗传变异。吊罗山保护区野生荔枝的遗传多样性参数为:P=61.1%,A=2.05,He=0.27。霸王岭居群野生荔枝的遗传多样性参数为:P=61.1%,A=2.05,He=0.28。两个群体的遗传分化极小,GST=0.040。居群间遗传一致度较高,I=0.977。共检测到8个稀有等位基因。两个自然保护区的野生荔枝均有各自特有的稀有等位基因,都应采取措施予以保护。Allozyme variation and genetic diversity were evaluated among wild litchis sampling from two nature protection areas of Bawangling and Diaoluoshan in Hainan Island. Eighteen loci of thirteen allozyme systems were detected and used for population genetic analysis. The results showed that seven locally rare alleles were found in the two wild litchi populations in the study. A high level of genetic diversity was detected in the two wild litchi populations, with mean number of alleles per locus A=2.05, percentage of polymorphic loci P=61.1% in both populations, mean expected heterozygosity He=0.23 in Diaoluoshan populations, and He=0.28 in Bawangling populations. The Gsr was 0.040, I=0.977, suggesting that nearly 98% of the total genetic diversity was distributed within populations. The two litchi populations should be protected as there are locally rare alleles respectively.

关 键 词：野生荔枝 自然保护区 等位酶 遗传多样性 

分 类 号：S667.1[农业科学—果树学]