免疫酶法与ELISA法检测EB病毒VCA-IgA的比较  

Comparison of IEMA and ELISA Techniques for Measurement of EB Viral VCA-IgA

作　　者：阳茂春[1] 陆爱英[1] 李山[1] 李若林[1] 徐明辉[1] 李泰阶[1] 劳小霞[1] 秦雪[1] 

机构地区：[1]广西医科大学第一附属医院临床医学实验部,广西南宁530021

出　　处：《标记免疫分析与临床》2009年第2期93-95,共3页Labeled Immunoassays and Clinical Medicine

基　　金：广西卫生厅科研课题(Z2008158)

摘　　要：为探讨免疫酶法与ELISA在鼻咽癌诊断中的临床价值,检测鼻咽癌组患者与健康对照组血清EB病毒壳抗原抗体,并对两种检测方法的结果进行分析。结果表明:177例鼻咽癌患者中,免疫酶法检测EB-VCA-IgA阳性率高92.1%(163/177),而国产试剂和进口试剂ELISA法检测结果阳性率都较低,分别为65.0%(115/177)和67.2%(119/177),有显著性差异(P<0.005);在90名对照组血清EB-VCA-IgA,免疫酶法的阴性率为100%(90/90),ELISA法中的国产和进口试剂的阴性率分别为:98.9%(89/90)和95.6%(86/90),无显著性差异(P>0.05)。为此免疫酶法检测EB-VCA-IgA的灵敏度较高,更适合用于鼻咽癌的普查、协助诊断等,具有实际临床意义。To evaluate the clinical value of the immunoenzymatic assay （IEA） and ELISA in the diagnosis of nasopharyngeal cancer, the serum levels of EB-VCA-IgA in patients with nasopharyngeal cancer and healthy people were detected by the immunoenzymatic assay（IEMA） and ELISA. The results showed that the positive rate of EB-VCA-IgA in 177 patients with nasopharyngeal cancer measured by IEMA was significantly higher （92.1%） than those by domestic-made ELISA Kit （65.0%） and imported ELISA Kit （67.2%）. There were no significant differences between the negative rate of EB-VCA-IgA in 90 healthy persons measured by IEMA （100%）, by domestic-made ELISA Kit （98.9%） and by imported ELISA Kit （95.6%）. The IEMA was more sensitive than ELISA to detect the serum levels of EB-VCA-IgA. The IEMA might be more suitable for general investigation and assisting diagnosis of nasopharyngeal cancer.

关 键 词：鼻咽癌 EB病毒壳抗原抗体 免疫酶法 酶联免疫吸附法 

分 类 号：R446.6[医药卫生—诊断学]