血清镁测定候选参考方法的建立及其方法学评价  

Development and Validation of Candidate Reference Methods for Determination of Serum Magnesium Based on Flame Atomic Absorption Spectrometry

作　　者：王金英[1] 王清涛[1] 马怀安[2] 李惠玲[3] 徐国宾[4] 徐静[1] 贾慧敏[1] 童清[1] 

机构地区：[1]首都医科大学附属北京朝阳医院检验科,北京100020 [2]首都医科大学燕京医学院,北京101300 [3]首都医科大学附属北京朝阳医院职业病科,北京100043 [4]北京大学第一医院检验科,北京100034

出　　处：《标记免疫分析与临床》2009年第2期99-102,共4页Labeled Immunoassays and Clinical Medicine

基　　金：863计划资助项目(2006AA020909);十一五科技支撑计划资助项目(2007BAI05B09)

摘　　要：为建立血清镁测定的候选参考方法并对所建立的方法进行评价，采用火焰原子吸收光谱法，将血清用含有一定量的盐酸和氧化镧的稀释液准确稀释到100倍，进行分析与测定。方法学评价显示，重复试验批内不精密度（以变异系数CV表示）为0．41％（0．17％～0．89％）；批间CV为0．79％（0．63％-0．90％）；总CV为0．83％（0．69％-0．92％）；准确度偏倚小于1．5％；低、高两个水平的平均加标回收率分别为100．31％和99．70％；灵敏度和检出限分别为1．87×10^-3 mmol／L和0．0023mmol／L；线性范围为0．5—2．0mmol／L；测量不确定度为0．9859％。在规范操作和严格控制实验条件下，火焰原子吸收光谱法测定血清镁精密度和准确度较高，建议用其作为血清镁测定的候选参考方法。To establish and validate a candidate reference method for determination of serum magnesium based on flame atomic absorption spectrometry （FAAS） ,the samples of serum magnesium diluted by 100 folds at the presence of HCl and La2O3 were measured by AA6800 atomic absorption spectrophotometer. The results showed that the intra- and inter-assay coefficients of variation were 0.41% （0.17% -0.89% ） and 0.79% （0.63% -0.90% ） respectively, and the total imprecision was 0.83% （0.69% -0.92% ）. The Bias of assay was lower than 1.5%. The recovery rates of low and high level were 100.31% and 99.70% respectively, the sensitivity of assay was 1.87 - 10^-3mmol/L and the limit of detection was 0. 0023mmol/L. The applicable measurement range was 0.5 - 2.0mmol/L, and the expected uncertainty was 0. 9859%. The method for determination of serum magnesium based on FAAS is veryaccurate and precise; it might be regarded as a candidate reference method for the determination of total magnesium in serum.

关 键 词：火焰原子吸收光谱法 血清 镁 参考测量方法 方法学评价 

分 类 号：R446.11[医药卫生—诊断学]