八宝瑞生丸质量标准的研究  

Study on Quality Standard of Babao Ruisheng Pellet

作　　者：刘静[1] 姜文红[2] 张清波[2] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江省药品检验所,哈尔滨150001

出　　处：《中国药品标准》2009年第2期109-112,共4页Drug Standards of China

摘　　要：目的：建立八宝瑞生丸的质量标准。方法：采用TLC对处方中的延胡索、香附、山楂进行定性鉴别；采用HPLC法测定甘草苷的含量。色谱柱为Phenomenex C18（4．6mm×250mm，5μm）；流动相为乙腈-0．2％冰醋酸溶液（12：88），流速为1．0mL·min^-1；检测波长276nm。结果：鉴别方法专属性强，甘草苷进样量在0．02～0．39μg范围内线性关系良好（r=0．9999）；平均回收率为99．86％，RSD为0．9％：结论：方法灵敏、准确，重现性好，可作为八宝瑞生丸的质量控制方法：Objective：The quality standard for Babao Ruisheng Pellet was established. Methods： A TLC method was used for the qualitative identification of rhizoma corydalis, rhizoma cyperi, fructus crataegi and radix glycytthizae in the formula. The content of liquiritin was determined by an HPLC method with a Phenomenex Cis column （4.6 mm × 250 mm,5μm） and mobile phase of acetonitrile -0. 2% solution of glacial acetic acid（ 12： 88）. The flow rate was 1.0 mL·min^-1. The detection wavelength was 276 nmo Results： The identification study showed strong specificity. The linear relationship of liquiritin was good within the rang of 0. 019 552 × 0. 391 04 μg（ r = 0. 999 9）and average recovery was 99. 86% with RSD 0. 9%. Conclusions： The method was sensitive, accurate and reproducible. It could be used in the quality control of Babao Ruisheng Pellet.

关 键 词：八宝瑞生丸 甘草苷 质量标准 薄层色谱法 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]