淫羊藿多糖的酶法提取及其抗氧化作用的研究  

Extraction of epimedium polysaccharide in enzyme method and its effects on reducing the free radical oxygen

作　　者：苏瑛[1] 郑晓翠[2] 唐咏[2] 刘鹏举[2] 孙东[2] 

机构地区：[1]沈阳农业大学理学院,沈阳110161 [2]沈阳农业大学生物科学技术学院,沈阳110161

出　　处：《食品科技》2009年第4期200-203,共4页Food Science and Technology

基　　金：沈阳农业大学青年基金项目(2007020)

摘　　要：以淫羊藿干粉为材料,分别考察了料液比、酶加入量及酶作用时间对淫羊藿多糖提取率的影响,然后在此基础上采用3因素3水平的正交实验对提取条件进行优化;同时研究了淫羊藿多糖对O-2·和·OH的抑制作用。结果表明:淫羊藿多糖酶法提取的最优工艺参数为:提取时间1.5h,酶加入量为1.6%,料液比1:25;淫羊藿多糖具有清除羟基自由基和超氧阴离子自由基的作用。Through single-factor experiment and orthogonal experiment of 3 factors and 3 levels, the conditions for extraction of polysaccharide from epimedium in enzyme method were optimized, and the same time, studied its effect on raducing the free radical oxygen; The result showed that： the optimized extraction conditions for polysaccharide form epimedium were 1.5 hours of extracting time, 1.6% of the quantity of enzyme and 1：25 proportion of epimedium to solution; EPS could eliminate the hydroxyl free radical and the superoxide anion free radical.

关 键 词：淫羊藿 多糖 抗氧化 

分 类 号：R284.2[医药卫生—中药学] TS201.2[医药卫生—中医学]